Haloalkane dehalogenases other than LinB can contribute to the γ-hexachlorocyclohexane utilization

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F25%3A00082314" target="_blank" >RIV/00159816:_____/25:00082314 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/25:00141583

Výsledek na webu

<a href="https://academic.oup.com/bbb/article-abstract/89/6/898/8052007" target="_blank" >https://academic.oup.com/bbb/article-abstract/89/6/898/8052007</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/bbb/zbaf022" target="_blank" >10.1093/bbb/zbaf022</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Haloalkane dehalogenases other than LinB can contribute to the γ-hexachlorocyclohexane utilization

Popis výsledku v původním jazyce

Haloalkane dehalogenases (HLDs) convert halogenated compounds to corresponding alcohols by a simple hydrolytic mechanism. Although many bacterial strains possess HLDs or HLD homologues, LinB is the only HLD known to be involved in the utilization of gamma-hexachlorocyclohexane (gamma-HCH), a man-made chlorinated pesticide. In this study, to gain insight into the functional evolution of HLDs toward gamma-HCH utilization, the linB gene in gamma-HCH-degrading Sphingobium japonicum strain UT26 was replaced by 7 other HLD or HLD homologous genes, including 3 putative ancestral enzymes. Interestingly, strains carrying genes for DmmA from a marine metagenome or Rluc_anc, an ancestor of Renilla-luciferin 2-monooxygenase and LinB, produced 2,5-dichlorophenol and 2,5-dichlorohydroquinone from gamma-HCH, which are indicators of LinB activity, and grew in minimal medium supplied with gamma-HCH as a sole carbon source. These results indicated that other HLDs, in addition to LinB, can play roles in the gamma-HCH utilization.

Název v anglickém jazyce

Haloalkane dehalogenases other than LinB can contribute to the γ-hexachlorocyclohexane utilization

Popis výsledku anglicky

Haloalkane dehalogenases (HLDs) convert halogenated compounds to corresponding alcohols by a simple hydrolytic mechanism. Although many bacterial strains possess HLDs or HLD homologues, LinB is the only HLD known to be involved in the utilization of gamma-hexachlorocyclohexane (gamma-HCH), a man-made chlorinated pesticide. In this study, to gain insight into the functional evolution of HLDs toward gamma-HCH utilization, the linB gene in gamma-HCH-degrading Sphingobium japonicum strain UT26 was replaced by 7 other HLD or HLD homologous genes, including 3 putative ancestral enzymes. Interestingly, strains carrying genes for DmmA from a marine metagenome or Rluc_anc, an ancestor of Renilla-luciferin 2-monooxygenase and LinB, produced 2,5-dichlorophenol and 2,5-dichlorohydroquinone from gamma-HCH, which are indicators of LinB activity, and grew in minimal medium supplied with gamma-HCH as a sole carbon source. These results indicated that other HLDs, in addition to LinB, can play roles in the gamma-HCH utilization.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY

ISSN

0916-8451

e-ISSN

1347-6947

Svazek periodika

89

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

JP - Japonsko

Počet stran výsledku

12

Strana od-do

898-909

Kód UT WoS článku

001457346000001

EID výsledku v databázi Scopus

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