3D endothelial network formation in hydrogels improved by stromal cells and specific growth factors

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F25%3A00082366" target="_blank" >RIV/00159816:_____/25:00082366 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14110/25:00143156

Výsledek na webu

<a href="https://www.nature.com/articles/s41598-025-25381-x" target="_blank" >https://www.nature.com/articles/s41598-025-25381-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-025-25381-x" target="_blank" >10.1038/s41598-025-25381-x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

3D endothelial network formation in hydrogels improved by stromal cells and specific growth factors

Popis výsledku v původním jazyce

The limiting factor in the current state-of-the-art bioengineering of human tissues/organoids in vitro is the lack of functional vasculature that would support growth, metabolic waste removal, and oxygen supply of the formed constructs. The three-dimensional (3D) tissues can be formed by utilization of specific cell types, different hydrogels and scaffolds that provide a physical support, in addition to specific medium composition. Establishment of chemically defined xeno-free conditions is of great interest when keeping in mind the transition from basic research to therapeutic applications. In this study, we present the formation of vascular network of human umbilical vein endothelial cells co-cultured with stromal cells (SCs), human dental pulp SCs or human adipose SCs, in natural hydrogels (Matrigel, GelMA) as well as xeno-free VitroGel hydrogels. As medium composition plays a pivotal role in the successful generation of biological constructs in vitro, we evaluated the potency of specific growth factors (GFs) to induce vascular network formation in 3D hydrogels. We confirmed that vascular endothelial growth factor (VEGF), the most used pro-angiogenic factor, is not mandatory in culture medium for vascular network formation. Rather the endothelial cell (EC) network in co-culture of ECs and SCs can be formed through stimulation of SCs with GFs such as insulin-like growth factor 1(IGF1), basic fibroblast growth factor (FGF2), and epidermal growth factor (EGF) regardless of serum presence in the medium. Also, we tested completely chemically defined medium that, when used together with xeno-free hydrogel, presents a step forward to application of such tissue constructs in translational regenerative medicine.

Název v anglickém jazyce

3D endothelial network formation in hydrogels improved by stromal cells and specific growth factors

Popis výsledku anglicky

The limiting factor in the current state-of-the-art bioengineering of human tissues/organoids in vitro is the lack of functional vasculature that would support growth, metabolic waste removal, and oxygen supply of the formed constructs. The three-dimensional (3D) tissues can be formed by utilization of specific cell types, different hydrogels and scaffolds that provide a physical support, in addition to specific medium composition. Establishment of chemically defined xeno-free conditions is of great interest when keeping in mind the transition from basic research to therapeutic applications. In this study, we present the formation of vascular network of human umbilical vein endothelial cells co-cultured with stromal cells (SCs), human dental pulp SCs or human adipose SCs, in natural hydrogels (Matrigel, GelMA) as well as xeno-free VitroGel hydrogels. As medium composition plays a pivotal role in the successful generation of biological constructs in vitro, we evaluated the potency of specific growth factors (GFs) to induce vascular network formation in 3D hydrogels. We confirmed that vascular endothelial growth factor (VEGF), the most used pro-angiogenic factor, is not mandatory in culture medium for vascular network formation. Rather the endothelial cell (EC) network in co-culture of ECs and SCs can be formed through stimulation of SCs with GFs such as insulin-like growth factor 1(IGF1), basic fibroblast growth factor (FGF2), and epidermal growth factor (EGF) regardless of serum presence in the medium. Also, we tested completely chemically defined medium that, when used together with xeno-free hydrogel, presents a step forward to application of such tissue constructs in translational regenerative medicine.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientific Reports

ISSN

2045-2322

e-ISSN

—

Svazek periodika

15

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

17

Strana od-do

nestránkováno

Kód UT WoS článku

001622418700036

EID výsledku v databázi Scopus

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