TYRAY-functionalized alginate bioinks for 3D bioprinting support stem cell culture and endothelial network formation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F25%3A00643019" target="_blank" >RIV/61389013:_____/25:00643019 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14110/25:00143608 RIV/65269705:_____/25:00083077 RIV/00159816:_____/25:00082287

Výsledek na webu

<a href="https://pubs.acs.org/doi/10.1021/acsbiomaterials.5c01132" target="_blank" >https://pubs.acs.org/doi/10.1021/acsbiomaterials.5c01132</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acsbiomaterials.5c01132" target="_blank" >10.1021/acsbiomaterials.5c01132</a>

Jazyk výsledku

angličtina

Název v původním jazyce

TYRAY-functionalized alginate bioinks for 3D bioprinting support stem cell culture and endothelial network formation

Popis výsledku v původním jazyce

3D bioprinting is transforming tissue engineering by enabling spatial arrangement of cells and cell aggregates within supportive hydrogels. Among available materials, alginate remains widely used for its biocompatibility, printability, and cost-effectiveness. However, its bioinert nature and lack of adhesive moieties restrict its capacity to support essential processes like cell adhesion, migration, and proliferation. In this study, we propose a comprehensive approach to enhance alginate hydrogels focusing on stem, stromal, and endothelial cell types to support extended growth and vascular network formation. Key innovations include the incorporation of the TYRAY peptide in 3D alginate hydrogels─its first application in this context─to promote cell adhesion and migration, accompanied by Ca(OH)2-modified surfaces for stable hydrogel anchoring and an ultrasonic mist cross-linking to preserve 3D structure fidelity. Functionalization with the TYRAY peptide significantly enhanced cell proliferation, promoted multicellular spheroid fusion, and supported endothelial network development in comparative culture setting. Together, these findings establish this defined, xeno-free alginate system as a versatile bioink material suitable for 3D culture and bioprinting applications.

Název v anglickém jazyce

TYRAY-functionalized alginate bioinks for 3D bioprinting support stem cell culture and endothelial network formation

Popis výsledku anglicky

3D bioprinting is transforming tissue engineering by enabling spatial arrangement of cells and cell aggregates within supportive hydrogels. Among available materials, alginate remains widely used for its biocompatibility, printability, and cost-effectiveness. However, its bioinert nature and lack of adhesive moieties restrict its capacity to support essential processes like cell adhesion, migration, and proliferation. In this study, we propose a comprehensive approach to enhance alginate hydrogels focusing on stem, stromal, and endothelial cell types to support extended growth and vascular network formation. Key innovations include the incorporation of the TYRAY peptide in 3D alginate hydrogels─its first application in this context─to promote cell adhesion and migration, accompanied by Ca(OH)2-modified surfaces for stable hydrogel anchoring and an ultrasonic mist cross-linking to preserve 3D structure fidelity. Functionalization with the TYRAY peptide significantly enhanced cell proliferation, promoted multicellular spheroid fusion, and supported endothelial network development in comparative culture setting. Together, these findings establish this defined, xeno-free alginate system as a versatile bioink material suitable for 3D culture and bioprinting applications.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10404 - Polymer science

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ACS Biomaterials Science & Engineering

ISSN

2373-9878

e-ISSN

2373-9878

Svazek periodika

11

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

16

Strana od-do

7368-7383

Kód UT WoS článku

001619486600001

EID výsledku v databázi Scopus

2-s2.0-105024260754