Dental pulp stem cells and their characterization

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F09%3A00201755" target="_blank" >RIV/00179906:_____/09:00201755 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11150/09:00208772

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Dental pulp stem cells and their characterization

Popis výsledku v původním jazyce

We isolated and expanded 16 lines of DPSCs in three different media over 40 population doublings. DPSCs cultivated in all media were cytogenetically stable and showed no signs of spontaneous differentiation. Over the entire cultivation period, we observed no changes in cell viability. Medium containing 2 % FCS supplemented with ITS provided better cultivation conditions for DPSCs (shorter DT and more stable proliferation activity were measured) than other tested media (e.g. most cited 10 % FCS or high FCS concentration media). Decrease in concentration of FCS and adding ITS into media had no negative effects on basics biological characteristics(viability, cell diameter). DPSCs cultivated in 2 % FCS with ITS showed lower positivity mesenchymal stem cell markers and HLA I compared to DPSCs cultivated in 2 % FCS or 10 % FCS media.

Název v anglickém jazyce

Dental pulp stem cells and their characterization

Popis výsledku anglicky

We isolated and expanded 16 lines of DPSCs in three different media over 40 population doublings. DPSCs cultivated in all media were cytogenetically stable and showed no signs of spontaneous differentiation. Over the entire cultivation period, we observed no changes in cell viability. Medium containing 2 % FCS supplemented with ITS provided better cultivation conditions for DPSCs (shorter DT and more stable proliferation activity were measured) than other tested media (e.g. most cited 10 % FCS or high FCS concentration media). Decrease in concentration of FCS and adding ITS into media had no negative effects on basics biological characteristics(viability, cell diameter). DPSCs cultivated in 2 % FCS with ITS showed lower positivity mesenchymal stem cell markers and HLA I compared to DPSCs cultivated in 2 % FCS or 10 % FCS media.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

30106 - Anatomy and morphology (plant science to be 1.6)

Projekt

<a href="/cs/project/NR9182" target="_blank" >NR9182: Izolace, kultivace, diferenciace a fenotypizace kmenových buněk zubní pulpy</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biomedical Papers

ISSN

1213-8118

e-ISSN

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Svazek periodika

153

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

6

Strana od-do

31-36

Kód UT WoS článku

000265639300006

EID výsledku v databázi Scopus

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