Human Plasma and Human Platelet-rich Plasma as a Substitute for Fetal Calf Serum during Long-term Cultivation of Mesenchymal Dental Pulp Stem Cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F14%3A10283985" target="_blank" >RIV/00179906:_____/14:10283985 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11150/14:10283985

Výsledek na webu

<a href="http://orbis.lfhk.cuni.cz/Acta_Medica/2014/2014_119.pdf" target="_blank" >http://orbis.lfhk.cuni.cz/Acta_Medica/2014/2014_119.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.14712/18059694.2014.50" target="_blank" >10.14712/18059694.2014.50</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Human Plasma and Human Platelet-rich Plasma as a Substitute for Fetal Calf Serum during Long-term Cultivation of Mesenchymal Dental Pulp Stem Cells

Popis výsledku v původním jazyce

Our aims were to isolate and cultivate mesenchymal dental pulp stem cells (DPSC) in various media enriched with human blood derivatives, and subsequently to investigate their basic biological properties. Methods: DPSC were cultivated in five different media based on ? MEM containing different concentrations of human plasma (HP), platelet-rich plasma (PRP), or fetal calf serum (FCS). The DPSC biological properties were examined periodically. Results: We cultivated DPSC in the various cultivation media over 15 population doublings except for the medium supplemented with 10% HP. Our results showed that DPSC cultivated in medium supplemented with 10% PRP showed the shortest average population doubling time (DT) (28.6 +- 4.6 hours), in contrast to DPSC cultivated in 10% HP which indicated the longest DT (156.2 +- 17.8 hours); hence this part of the experiment had been cancelled in the 6th passage. DPSC cultivated in media with 2% FCS+ITS (DT 47.3 +- 10.4 hours), 2% PRP (DT 40.1 +- 5.7 hours

Název v anglickém jazyce

Human Plasma and Human Platelet-rich Plasma as a Substitute for Fetal Calf Serum during Long-term Cultivation of Mesenchymal Dental Pulp Stem Cells

Popis výsledku anglicky

Our aims were to isolate and cultivate mesenchymal dental pulp stem cells (DPSC) in various media enriched with human blood derivatives, and subsequently to investigate their basic biological properties. Methods: DPSC were cultivated in five different media based on ? MEM containing different concentrations of human plasma (HP), platelet-rich plasma (PRP), or fetal calf serum (FCS). The DPSC biological properties were examined periodically. Results: We cultivated DPSC in the various cultivation media over 15 population doublings except for the medium supplemented with 10% HP. Our results showed that DPSC cultivated in medium supplemented with 10% PRP showed the shortest average population doubling time (DT) (28.6 +- 4.6 hours), in contrast to DPSC cultivated in 10% HP which indicated the longest DT (156.2 +- 17.8 hours); hence this part of the experiment had been cancelled in the 6th passage. DPSC cultivated in media with 2% FCS+ITS (DT 47.3 +- 10.4 hours), 2% PRP (DT 40.1 +- 5.7 hours

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FF - ORL, oftalmologie, stomatologie

OECD FORD obor

—

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Medica (Hradec Králové)

ISSN

1211-4286

e-ISSN

—

Svazek periodika

57

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

8

Strana od-do

119-126

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—