The transcription factor GATA-4 regulates cytochrome P4502C19 gene expression

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F10%3A10080541" target="_blank" >RIV/00179906:_____/10:10080541 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The transcription factor GATA-4 regulates cytochrome P4502C19 gene expression

Popis výsledku v původním jazyce

Cytochrome P450 2C19 (CYP2C19) is an important enzyme involved in the metabolism of antiulcer drugs and antidepressants. However, despite the well documented drug-dependent variability of CYP2C19 expression, the mechanisms underlying the regulation of the enzyme remain unknown. In this study we investigated whether the transcription factor family GATA is involved in the regulation of CYP2C19 gene expression. We identified a novel putative GATA binding site at position -165/-156 within the CYP2C19 gene promoter and performed luciferase gene reporter assays, electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitations (ChIP) to document a sequence-specific interaction of GATA proteins with this putative GATA binding site. The data obtained indicate that GATA-4 plays an important role in the transcriptional regulation of CYP2C19 expression. 10.1016/j.lfs.2010.02.021

Název v anglickém jazyce

The transcription factor GATA-4 regulates cytochrome P4502C19 gene expression

Popis výsledku anglicky

Cytochrome P450 2C19 (CYP2C19) is an important enzyme involved in the metabolism of antiulcer drugs and antidepressants. However, despite the well documented drug-dependent variability of CYP2C19 expression, the mechanisms underlying the regulation of the enzyme remain unknown. In this study we investigated whether the transcription factor family GATA is involved in the regulation of CYP2C19 gene expression. We identified a novel putative GATA binding site at position -165/-156 within the CYP2C19 gene promoter and performed luciferase gene reporter assays, electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitations (ChIP) to document a sequence-specific interaction of GATA proteins with this putative GATA binding site. The data obtained indicate that GATA-4 plays an important role in the transcriptional regulation of CYP2C19 expression. 10.1016/j.lfs.2010.02.021

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

N - Vyzkumna aktivita podporovana z neverejnych zdroju

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Life Sciences

ISSN

0024-3205

e-ISSN

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Svazek periodika

2010 (86)

Číslo periodika v rámci svazku

19-20

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

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Kód UT WoS článku

000277192500001

EID výsledku v databázi Scopus

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