Genotyping of varicella-zoster virus (VZV) wild-type strains isolated in the Czech Republic
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F11%3A10107427" target="_blank" >RIV/00179906:_____/11:10107427 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00216208:11150/11:10107427
Výsledek na webu
<a href="http://biomed.papers.upol.cz/pdfs/bio/2011/04/11.pdf" target="_blank" >http://biomed.papers.upol.cz/pdfs/bio/2011/04/11.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.5507/bp.2011.056" target="_blank" >10.5507/bp.2011.056</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Genotyping of varicella-zoster virus (VZV) wild-type strains isolated in the Czech Republic
Popis výsledku v původním jazyce
A total of 273 clinical samples were obtained from patients exhibiting symptoms of varicella zoster virus (VZV) infection manifested as chickenpox or herpes zoster (HZ) treated in the Faculty Hospital of Charles University, Medical School in Hradec Kralove, Czech Republic Characterization of individual VZV strains was performed utilizing restriction fragment length polymorphisms (RFLPs), PCR and sequencing. Single nucleotide polymorphisms (SNP) in the open reading frames (ORF) 21, 22 and 50 were used toidentify individual VZV strains. All clinical isolates (97 from varicella, 176 from herpes zoster) were VZV positive wild-type strains. Sequencing analysis showed that 89 isolates were of the European E1 genotype, 180 were of the European E2 genotype and 2 were identified as the Mosaic M1 strain. In addition, two unusual genotypes were identified, both representing a combination of E1 and M2 strain specific SNPs. Our prospective VZV genotyping study is the first one monitoring the situa
Název v anglickém jazyce
Genotyping of varicella-zoster virus (VZV) wild-type strains isolated in the Czech Republic
Popis výsledku anglicky
A total of 273 clinical samples were obtained from patients exhibiting symptoms of varicella zoster virus (VZV) infection manifested as chickenpox or herpes zoster (HZ) treated in the Faculty Hospital of Charles University, Medical School in Hradec Kralove, Czech Republic Characterization of individual VZV strains was performed utilizing restriction fragment length polymorphisms (RFLPs), PCR and sequencing. Single nucleotide polymorphisms (SNP) in the open reading frames (ORF) 21, 22 and 50 were used toidentify individual VZV strains. All clinical isolates (97 from varicella, 176 from herpes zoster) were VZV positive wild-type strains. Sequencing analysis showed that 89 isolates were of the European E1 genotype, 180 were of the European E2 genotype and 2 were identified as the Mosaic M1 strain. In addition, two unusual genotypes were identified, both representing a combination of E1 and M2 strain specific SNPs. Our prospective VZV genotyping study is the first one monitoring the situa
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EE - Mikrobiologie, virologie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2011
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, CzechoSK
ISSN
1213-8118
e-ISSN
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Svazek periodika
155
Číslo periodika v rámci svazku
4
Stát vydavatele periodika
CZ - Česká republika
Počet stran výsledku
6
Strana od-do
379-384
Kód UT WoS článku
000299697000012
EID výsledku v databázi Scopus
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