Genotyping of varicella zoster virus wild-type strains isolated in Czech republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F11%3A00002431" target="_blank" >RIV/60162694:G44__/11:00002431 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.5507/bp.2011.056" target="_blank" >http://dx.doi.org/10.5507/bp.2011.056</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Genotyping of varicella zoster virus wild-type strains isolated in Czech republic

Popis výsledku v původním jazyce

A total of 273 clinical samples were obtained from patients exhibiting symptoms of varicella zoster virus (VZV) infection manifested as chickenpox or herpes zoster (HZ) treated in the Faculty Hospital of Charles University, Medical School in Hradec Kralove, Czech Republic. Characterization of individual VZV strains was performed utilizing restriction fragment length polymorphisms (RFLPs), PCR and sequencing. Single nucleotide polymorphisms (SNP) in the open reading frames (ORF) 21, 22 and 50 were used to identify individual VZV strains. All clinical isolates (97 from varicella, 176 from herpes zoster) were VZV positive wild-type strains. Sequencing analysis showed that 89 isolates were of the European E1 genotype, 180 were of the European E2 genotype and 2 were identified as the Mosaic M1 strain. In addition, two unusual genotypes were identified, both representing a combination of E1 and M2 strain specific SNPs. Our prospective VZV genotyping study is the first one monitoring the situ

Název v anglickém jazyce

Genotyping of varicella zoster virus wild-type strains isolated in Czech republic

Popis výsledku anglicky

A total of 273 clinical samples were obtained from patients exhibiting symptoms of varicella zoster virus (VZV) infection manifested as chickenpox or herpes zoster (HZ) treated in the Faculty Hospital of Charles University, Medical School in Hradec Kralove, Czech Republic. Characterization of individual VZV strains was performed utilizing restriction fragment length polymorphisms (RFLPs), PCR and sequencing. Single nucleotide polymorphisms (SNP) in the open reading frames (ORF) 21, 22 and 50 were used to identify individual VZV strains. All clinical isolates (97 from varicella, 176 from herpes zoster) were VZV positive wild-type strains. Sequencing analysis showed that 89 isolates were of the European E1 genotype, 180 were of the European E2 genotype and 2 were identified as the Mosaic M1 strain. In addition, two unusual genotypes were identified, both representing a combination of E1 and M2 strain specific SNPs. Our prospective VZV genotyping study is the first one monitoring the situ

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FN - Epidemiologie, infekční nemoci a klinická imunologie

OECD FORD obor

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Projekt

<a href="/cs/project/GAP304%2F10%2F1161" target="_blank" >GAP304/10/1161: Role buněčných proteinů asociovaných s viry v patogenezi dysfunkce T lymfocytů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biomedical Papers

ISSN

1213-8118

e-ISSN

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Svazek periodika

155

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

6

Strana od-do

265-270

Kód UT WoS článku

000299697000012

EID výsledku v databázi Scopus

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