Effects of amlodipine on bone metabolism in male albino Wistar rats

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F11%3A10124061" target="_blank" >RIV/00179906:_____/11:10124061 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60162694:G44__/11:43874773 RIV/00216208:11150/11:10124061

Výsledek na webu

<a href="http://actavet.vfu.cz/pdf/201180040391.pdf" target="_blank" >http://actavet.vfu.cz/pdf/201180040391.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2754/avb201180040391" target="_blank" >10.2754/avb201180040391</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effects of amlodipine on bone metabolism in male albino Wistar rats

Popis výsledku v původním jazyce

The aim of our study was to investigate the effect of amlodipine on bone metabolism in male rats. Amlodipine (0.3 mg/100 g BW; gavage) was administered to 8 rats for 8 weeks. Control group (n=8) received aqua pro inj.. Bone marker concentrations of CTX-I and PINP in serum, and BALP in both serum and bone homogenate were measured by ELISA. We investigated expression of BMP-2 in tibia using Western blot, and bone mineral density was measured by DXA in lumbar and caudal vertebrae and in femoral areas. Femurs were used for biomechanical testing. After 8 weeks of amlodipine administration there was decrease in serum levels of BALP (p=0.0009) and CTX-I (p=0.003), and level of BALP in bone homogenate (p=0.026) compared to controls. Expression of BMP-2 was increased after amlodipine administration. Our findings suggest that amlodipine has retarding influence on bone metabolism in rats by decreasing bone turnover, which probably in consequence increases expression of BMP-2.

Název v anglickém jazyce

Effects of amlodipine on bone metabolism in male albino Wistar rats

Popis výsledku anglicky

The aim of our study was to investigate the effect of amlodipine on bone metabolism in male rats. Amlodipine (0.3 mg/100 g BW; gavage) was administered to 8 rats for 8 weeks. Control group (n=8) received aqua pro inj.. Bone marker concentrations of CTX-I and PINP in serum, and BALP in both serum and bone homogenate were measured by ELISA. We investigated expression of BMP-2 in tibia using Western blot, and bone mineral density was measured by DXA in lumbar and caudal vertebrae and in femoral areas. Femurs were used for biomechanical testing. After 8 weeks of amlodipine administration there was decrease in serum levels of BALP (p=0.0009) and CTX-I (p=0.003), and level of BALP in bone homogenate (p=0.026) compared to controls. Expression of BMP-2 was increased after amlodipine administration. Our findings suggest that amlodipine has retarding influence on bone metabolism in rats by decreasing bone turnover, which probably in consequence increases expression of BMP-2.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Veterinaria Brno

ISSN

0001-7213

e-ISSN

—

Svazek periodika

80

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

6

Strana od-do

391-396

Kód UT WoS článku

000301811400012

EID výsledku v databázi Scopus

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