Organic Cation Transporter 3 (OCT3/SLC22A3) and Multidrug and Toxin Extrusion 1 (MATE1/SLC47A1) Transporter in the Placenta and Fetal Tissues: Expression Profile and Fetus Protective Role at Different Stages of Gestation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F13%3A10146015" target="_blank" >RIV/00179906:_____/13:10146015 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11160/13:10146015 RIV/00216208:11150/13:10146015

Výsledek na webu

<a href="http://www.biolreprod.org/content/88/3/55.full" target="_blank" >http://www.biolreprod.org/content/88/3/55.full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1095/biolreprod.112.105064" target="_blank" >10.1095/biolreprod.112.105064</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Organic Cation Transporter 3 (OCT3/SLC22A3) and Multidrug and Toxin Extrusion 1 (MATE1/SLC47A1) Transporter in the Placenta and Fetal Tissues: Expression Profile and Fetus Protective Role at Different Stages of Gestation

Popis výsledku v původním jazyce

In this study, we hypothesized that changes in placental levels of OCT3 and MATE1 throughout gestation might affect the fetal protection and detoxification. Using quantitative RT-PCR, Western blot analysis, and immunohistochemistry, we were able to detect Oct3/OCT3 and Mate1/MATE1 expression in the rat placenta as early as on Gestation Day (gd) 12 with increasing tendency toward the end of pregnancy. Contrary to the current literature, we were able to detect also MATE1/MATE2 isoforms in the human placenta. We also investigated the protective function of the placenta against organic cations at different gds. We conclude that increased expression of placental OCT3 and MATE1 along with general maturation of the placental tissues results in significantly lower transport of organic cations (MPP+) from mother to fetus. In contrast, decreasing expression of OCT3 and MATE1 in human placenta indicates these transporters may play a role in fetal protection preferentially at earlier stages of ges

Název v anglickém jazyce

Organic Cation Transporter 3 (OCT3/SLC22A3) and Multidrug and Toxin Extrusion 1 (MATE1/SLC47A1) Transporter in the Placenta and Fetal Tissues: Expression Profile and Fetus Protective Role at Different Stages of Gestation

Popis výsledku anglicky

In this study, we hypothesized that changes in placental levels of OCT3 and MATE1 throughout gestation might affect the fetal protection and detoxification. Using quantitative RT-PCR, Western blot analysis, and immunohistochemistry, we were able to detect Oct3/OCT3 and Mate1/MATE1 expression in the rat placenta as early as on Gestation Day (gd) 12 with increasing tendency toward the end of pregnancy. Contrary to the current literature, we were able to detect also MATE1/MATE2 isoforms in the human placenta. We also investigated the protective function of the placenta against organic cations at different gds. We conclude that increased expression of placental OCT3 and MATE1 along with general maturation of the placental tissues results in significantly lower transport of organic cations (MPP+) from mother to fetus. In contrast, decreasing expression of OCT3 and MATE1 in human placenta indicates these transporters may play a role in fetal protection preferentially at earlier stages of ges

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FR - Farmakologie a lékárnická chemie

OECD FORD obor

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Projekt

<a href="/cs/project/GAP303%2F12%2F0850" target="_blank" >GAP303/12/0850: Transplacentární farmakokinetika antiretrovirálních léčiv; interakce s efluxními transportéry léčiv</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biology of Reproduction

ISSN

0006-3363

e-ISSN

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Svazek periodika

88

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

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Kód UT WoS článku

000315958900002

EID výsledku v databázi Scopus

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