New approach for the clinical monitoring of 25-hydroxyvitamin D-3 and 25-hydroxyvitamin D-2 by ultra high performance liquid chromatography with MS/MS based on the standard reference material 972

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F13%3A10191186" target="_blank" >RIV/00179906:_____/13:10191186 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11150/13:10191186 RIV/00216208:11160/13:10191186

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1002/jssc.201300553/full" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/jssc.201300553/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.201300553" target="_blank" >10.1002/jssc.201300553</a>

Jazyk výsledku

angličtina

Název v původním jazyce

New approach for the clinical monitoring of 25-hydroxyvitamin D-3 and 25-hydroxyvitamin D-2 by ultra high performance liquid chromatography with MS/MS based on the standard reference material 972

Popis výsledku v původním jazyce

Biomarkers, 25-hydroxyvitamin D-3 and 25-hydroxyvitamin D-2, are important indicators of the vitamin D general status and are monitored in several pathophysiological disorders, such as osteoporosis, diabetes, heart disease, etc. A novel ultra-HPLC with MS/MS methodology for the analysis of 25-hydroxyvitamin D derivatives coupled with a very simple and highly rapid sample preparation step was developed. Analytical parameters obtained showed linearity (R-2) above 0.999 for both vitamins with accuracies between 95.8 and 102%. The LODs were as low as 0.22 and 0.67 nmol/L for 25-hydroxyvitamin D-3 and 25-hydroxyvitamin D-2, respectively. Intra-assay precision (%RSD) was lower than 4.5%, and inter-assay precision (%RSD) was lower than 6.5%. The feasibility of the developed methodology to be applied in clinical routine analysis has been proved by its application in blood samples from non-agenarian patients, patients with familial hypercholesterolemia and patients suffering from age-related ma

Název v anglickém jazyce

New approach for the clinical monitoring of 25-hydroxyvitamin D-3 and 25-hydroxyvitamin D-2 by ultra high performance liquid chromatography with MS/MS based on the standard reference material 972

Popis výsledku anglicky

Biomarkers, 25-hydroxyvitamin D-3 and 25-hydroxyvitamin D-2, are important indicators of the vitamin D general status and are monitored in several pathophysiological disorders, such as osteoporosis, diabetes, heart disease, etc. A novel ultra-HPLC with MS/MS methodology for the analysis of 25-hydroxyvitamin D derivatives coupled with a very simple and highly rapid sample preparation step was developed. Analytical parameters obtained showed linearity (R-2) above 0.999 for both vitamins with accuracies between 95.8 and 102%. The LODs were as low as 0.22 and 0.67 nmol/L for 25-hydroxyvitamin D-3 and 25-hydroxyvitamin D-2, respectively. Intra-assay precision (%RSD) was lower than 4.5%, and inter-assay precision (%RSD) was lower than 6.5%. The feasibility of the developed methodology to be applied in clinical routine analysis has been proved by its application in blood samples from non-agenarian patients, patients with familial hypercholesterolemia and patients suffering from age-related ma

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FB - Endokrinologie, diabetologie, metabolismus, výživa

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Separation Science

ISSN

1615-9306

e-ISSN

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Svazek periodika

36

Číslo periodika v rámci svazku

23

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

7

Strana od-do

3702-3708

Kód UT WoS článku

000327556300008

EID výsledku v databázi Scopus

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