A New Concept in the Separation of Polyethyleneglycol-Modified PAMAM Dendrons by Liquid Chromatography

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F14%3A10281336" target="_blank" >RIV/00179906:_____/14:10281336 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62690094:18470/14:50002316 RIV/44555601:13440/14:43885504

Výsledek na webu

<a href="http://dx.doi.org/10.1093/chromsci/bmt032" target="_blank" >http://dx.doi.org/10.1093/chromsci/bmt032</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/chromsci/bmt032" target="_blank" >10.1093/chromsci/bmt032</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A New Concept in the Separation of Polyethyleneglycol-Modified PAMAM Dendrons by Liquid Chromatography

Popis výsledku v původním jazyce

In this work, a unique high-performance liquid chromatographic method was developed and applied for monitoring the synthesis of polyethyleneglycol surface modified poly(amidoamine) cystamine core dendrimers (PEG-PAMAMs) and PEG-PAMAM-alkynes with a single alkyne moiety attached to the core of a dendron through a unique sulfhydryl group. The separation of the products was performed on a column with a pentafluorphenylpropyl stationary phase, allowing multiple mechanisms of selectivity. More than 50 peakswere separated in one run, reflecting the degree of dendrimer PEGylation (PEG average molecular mass: 3,000). Moreover, modification of PAMAM with a single alkyne group could be distinguished. The developed method can be used for the general characterization and separation of PAMAM derivatives, in which the degree of modification is critical for final applications.

Název v anglickém jazyce

A New Concept in the Separation of Polyethyleneglycol-Modified PAMAM Dendrons by Liquid Chromatography

Popis výsledku anglicky

In this work, a unique high-performance liquid chromatographic method was developed and applied for monitoring the synthesis of polyethyleneglycol surface modified poly(amidoamine) cystamine core dendrimers (PEG-PAMAMs) and PEG-PAMAM-alkynes with a single alkyne moiety attached to the core of a dendron through a unique sulfhydryl group. The separation of the products was performed on a column with a pentafluorphenylpropyl stationary phase, allowing multiple mechanisms of selectivity. More than 50 peakswere separated in one run, reflecting the degree of dendrimer PEGylation (PEG average molecular mass: 3,000). Moreover, modification of PAMAM with a single alkyne group could be distinguished. The developed method can be used for the general characterization and separation of PAMAM derivatives, in which the degree of modification is critical for final applications.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

—

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatographic Science

ISSN

0021-9665

e-ISSN

—

Svazek periodika

52

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

321-328

Kód UT WoS článku

000342734400006

EID výsledku v databázi Scopus

—