History of cervical excisional treatment is associated with changes in the cervical microbiota in women with preterm prelabor rupture of membranes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F24%3A10488181" target="_blank" >RIV/00179906:_____/24:10488181 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11150/24:10488181

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=gOQ9rXbKzU" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=gOQ9rXbKzU</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/14767058.2024.2425761" target="_blank" >10.1080/14767058.2024.2425761</a>

Jazyk výsledku

angličtina

Název v původním jazyce

History of cervical excisional treatment is associated with changes in the cervical microbiota in women with preterm prelabor rupture of membranes

Popis výsledku v původním jazyce

Background: This study aimed to determine the differences in the cervical load and prevalence of Lactobacillus crispatus DNA, Lactobacillus iners DNA, Gardnerella vaginalis DNA, Sneathia sanguinegens DNA, and Ureaplasma species DNA between pregnant women with preterm prelabor rupture of membranes (PPROM) with and without a history of cervical excisional treatment. We also assessed the changes in the cervical load and prevalence of L. crispatus DNA, L. iners DNA, G. vaginalis DNA, S. sanguinegens DNA, and U. spp DNA. according to the cone length. Methods: This retrospective study included 132 women with singleton pregnancies complicated by PPROM. For all women, information about the cervical loads of bacterial DNA corresponding to L. crispatus, L. iners, G. vaginalis, S. sanguinegens, and U. spp., which was assessed using PCR, was available. Results: Women with a history of cervical excisional treatment had a higher cervical load of L. iners DNA (4.4 x 10(6) copies DNA/mL vs. 3.5 x 10(5) copies DNA/mL, p = .04) and a higher load and prevalence of U. spp. DNA (1.1 x 10(5) copies DNA/mL vs. 9.6 x 10(4) copies DNA/mL, p = .03; 2.7% vs. 0.5%, p = .04) than those without a history of cervical excisional treatment. In the subset of women with a history of cervical excisional treatment, those with a cone length 18 mm and more had a lower relative abundance of L. crispatus DNA (6% vs. 89%, p = .02), a higher load and relative abundance of L. iners DNA (1.1 x 10(7) copies DNA/mL vs. 8.2 x 10(5) copies DNA/mL, p = .04; 91% vs. 35%, p = .04), and higher loads of G. vaginalis DNA (7.6 x 10(4) copies DNA/mL vs. 3.2 x 10(2) copies DNA/mL, p = .02) than those with cone length &lt; 18 mm. Conclusions: A history of cervical excisional treatment was associated with alterations in the cervical microbiota composition in pregnant women with PPROM.

Název v anglickém jazyce

History of cervical excisional treatment is associated with changes in the cervical microbiota in women with preterm prelabor rupture of membranes

Popis výsledku anglicky

Background: This study aimed to determine the differences in the cervical load and prevalence of Lactobacillus crispatus DNA, Lactobacillus iners DNA, Gardnerella vaginalis DNA, Sneathia sanguinegens DNA, and Ureaplasma species DNA between pregnant women with preterm prelabor rupture of membranes (PPROM) with and without a history of cervical excisional treatment. We also assessed the changes in the cervical load and prevalence of L. crispatus DNA, L. iners DNA, G. vaginalis DNA, S. sanguinegens DNA, and U. spp DNA. according to the cone length. Methods: This retrospective study included 132 women with singleton pregnancies complicated by PPROM. For all women, information about the cervical loads of bacterial DNA corresponding to L. crispatus, L. iners, G. vaginalis, S. sanguinegens, and U. spp., which was assessed using PCR, was available. Results: Women with a history of cervical excisional treatment had a higher cervical load of L. iners DNA (4.4 x 10(6) copies DNA/mL vs. 3.5 x 10(5) copies DNA/mL, p = .04) and a higher load and prevalence of U. spp. DNA (1.1 x 10(5) copies DNA/mL vs. 9.6 x 10(4) copies DNA/mL, p = .03; 2.7% vs. 0.5%, p = .04) than those without a history of cervical excisional treatment. In the subset of women with a history of cervical excisional treatment, those with a cone length 18 mm and more had a lower relative abundance of L. crispatus DNA (6% vs. 89%, p = .02), a higher load and relative abundance of L. iners DNA (1.1 x 10(7) copies DNA/mL vs. 8.2 x 10(5) copies DNA/mL, p = .04; 91% vs. 35%, p = .04), and higher loads of G. vaginalis DNA (7.6 x 10(4) copies DNA/mL vs. 3.2 x 10(2) copies DNA/mL, p = .02) than those with cone length &lt; 18 mm. Conclusions: A history of cervical excisional treatment was associated with alterations in the cervical microbiota composition in pregnant women with PPROM.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30214 - Obstetrics and gynaecology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

The Journal of Maternal-Fetal &amp; Neonatal Medicine

ISSN

1476-7058

e-ISSN

1476-4954

Svazek periodika

37

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

2425761

Kód UT WoS článku

001355207800001

EID výsledku v databázi Scopus

2-s2.0-85209372331