Dried urine spot as a stable, green, and practical microsampling tool in clinical practice for quantification of neopterin and creatinine

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F25%3A10499513" target="_blank" >RIV/00179906:_____/25:10499513 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11160/25:10499513

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=wU9W7s1x0a" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=wU9W7s1x0a</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/d5gc00956a" target="_blank" >10.1039/d5gc00956a</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Dried urine spot as a stable, green, and practical microsampling tool in clinical practice for quantification of neopterin and creatinine

Popis výsledku v původním jazyce

Dried urine spot (DUS) is demonstrated to be a useful microsampling technique for neopterin and creatinine analysis. The method was verified using samples from 12 healthy volunteers, 12 cancer patients, and 12 patients infected with the SARS-CoV-2 virus and provided results comparable to the routine reference method. DUS samples were prepared using a simple dilute-and-shoot approach with a buffer solution prior to analysis. The developed method requires only 10 mu L samples, making it feasible for home sampling when combined with commercially available pre-cut DUS devices with volumetric microfluidics or capillary transfer tubes. The method is environmentally friendly as it minimizes reagent use, requires only 110 mu L phosphate buffer and no organic solvents. DUS also requires 250 times less storage space than liquid urine. The technique also improves laboratory work safety by minimizing the use of harmful extraction solvents. Dried samples are non-biohazardous and stable for up to 5 days at 40 degrees C and for 4 months at room temperature. This allows cost-effective transport via standard mail. The study also evaluated practical considerations for clinical laboratories and home sampling. Volumetric urine sampling is simple using capillary transfer tubes or commercially available volumetric microfluidic devices. When integrated with telemedicine, this microsampling approach can reduce unnecessary hospital visits for patients and improve patient access to clinical testing.

Název v anglickém jazyce

Dried urine spot as a stable, green, and practical microsampling tool in clinical practice for quantification of neopterin and creatinine

Popis výsledku anglicky

Dried urine spot (DUS) is demonstrated to be a useful microsampling technique for neopterin and creatinine analysis. The method was verified using samples from 12 healthy volunteers, 12 cancer patients, and 12 patients infected with the SARS-CoV-2 virus and provided results comparable to the routine reference method. DUS samples were prepared using a simple dilute-and-shoot approach with a buffer solution prior to analysis. The developed method requires only 10 mu L samples, making it feasible for home sampling when combined with commercially available pre-cut DUS devices with volumetric microfluidics or capillary transfer tubes. The method is environmentally friendly as it minimizes reagent use, requires only 110 mu L phosphate buffer and no organic solvents. DUS also requires 250 times less storage space than liquid urine. The technique also improves laboratory work safety by minimizing the use of harmful extraction solvents. Dried samples are non-biohazardous and stable for up to 5 days at 40 degrees C and for 4 months at room temperature. This allows cost-effective transport via standard mail. The study also evaluated practical considerations for clinical laboratories and home sampling. Volumetric urine sampling is simple using capillary transfer tubes or commercially available volumetric microfluidic devices. When integrated with telemedicine, this microsampling approach can reduce unnecessary hospital visits for patients and improve patient access to clinical testing.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30202 - Endocrinology and metabolism (including diabetes, hormones)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Green Chemistry

ISSN

1463-9262

e-ISSN

1463-9270

Svazek periodika

27

Číslo periodika v rámci svazku

36

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

14

Strana od-do

11007-11020

Kód UT WoS článku

001522484900001

EID výsledku v databázi Scopus

2-s2.0-105009945451