Volumetric dried blood spot microsampling: A sustainable, patient-friendly, and practical approach for retinol and alpha-tocopherol analysis in a clinical setting

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F25%3A10504947" target="_blank" >RIV/00179906:_____/25:10504947 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11160/25:10504947

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=_R-qUHvILD" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=_R-qUHvILD</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ymeth.2025.08.006" target="_blank" >10.1016/j.ymeth.2025.08.006</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Volumetric dried blood spot microsampling: A sustainable, patient-friendly, and practical approach for retinol and alpha-tocopherol analysis in a clinical setting

Popis výsledku v původním jazyce

Dried blood spot (DBS) technique has gained significant attention due to the growth of decentralized diagnostics. This technique reduces the number of hospital visits for patients and the workload for personnel in specialized hospitals. This microsampling method provides an environmentally friendly (green) and patient-friendly alternative to conventional phlebotomy. Challenges related to sample heterogeneity in traditional DBS cards have been overcome by the volumetric DBS sampling using new types of commercially available devices. Due to the unstable nature of the analytes, commercial volumetric DBS devices allow blood sampling at primary care units in remote settings and facilitate transport it via temperature-controlled systems. Blood sample stability has improved from 24 h at 4-8 degrees C to 30 days at -80 degrees C. DBS also requires over 1000 times less shipping and storage space than liquid blood. We optimized the DBS method to require only 10 mu L of blood and achieve extraction efficiencies of over 90 % for retinol when the result from validated method is the reference value. However, alpha-tocopherol recovery varied from 53 to 75 % depending on the filter paper type used. Furthermore, we successfully developed a liquid-liquid extraction method for both analytes from whole blood, with over 90 % recovery. Our approaches eliminate the need for separate serum and erythrocyte extractions, simplify sample preparation, and reduce reagent use and energy consumption. Both devices enable reliable volumetric collection. Our approach makes micronutrient monitoring more accessible and enables sample collection in decentralized settings. This aligns with the objectives of green analytical chemistry and universal health coverage.

Název v anglickém jazyce

Volumetric dried blood spot microsampling: A sustainable, patient-friendly, and practical approach for retinol and alpha-tocopherol analysis in a clinical setting

Popis výsledku anglicky

Dried blood spot (DBS) technique has gained significant attention due to the growth of decentralized diagnostics. This technique reduces the number of hospital visits for patients and the workload for personnel in specialized hospitals. This microsampling method provides an environmentally friendly (green) and patient-friendly alternative to conventional phlebotomy. Challenges related to sample heterogeneity in traditional DBS cards have been overcome by the volumetric DBS sampling using new types of commercially available devices. Due to the unstable nature of the analytes, commercial volumetric DBS devices allow blood sampling at primary care units in remote settings and facilitate transport it via temperature-controlled systems. Blood sample stability has improved from 24 h at 4-8 degrees C to 30 days at -80 degrees C. DBS also requires over 1000 times less shipping and storage space than liquid blood. We optimized the DBS method to require only 10 mu L of blood and achieve extraction efficiencies of over 90 % for retinol when the result from validated method is the reference value. However, alpha-tocopherol recovery varied from 53 to 75 % depending on the filter paper type used. Furthermore, we successfully developed a liquid-liquid extraction method for both analytes from whole blood, with over 90 % recovery. Our approaches eliminate the need for separate serum and erythrocyte extractions, simplify sample preparation, and reduce reagent use and energy consumption. Both devices enable reliable volumetric collection. Our approach makes micronutrient monitoring more accessible and enables sample collection in decentralized settings. This aligns with the objectives of green analytical chemistry and universal health coverage.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30104 - Pharmacology and pharmacy

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Methods

ISSN

1046-2023

e-ISSN

1095-9130

Svazek periodika

243

Číslo periodika v rámci svazku

NOV

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

40-48

Kód UT WoS článku

001561944800001

EID výsledku v databázi Scopus

2-s2.0-105014031175