Splicing Enhancers at Intron–Exon Borders Participate in Acceptor Splice Sites Recognition

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209775%3A_____%2F20%3AN0000009" target="_blank" >RIV/00209775:_____/20:N0000009 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.mdpi.com/1422-0067/21/18/6553" target="_blank" >https://www.mdpi.com/1422-0067/21/18/6553</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/ijms21186553" target="_blank" >10.3390/ijms21186553</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Splicing Enhancers at Intron–Exon Borders Participate in Acceptor Splice Sites Recognition

Popis výsledku v původním jazyce

Acceptor splice site recognition (3´ splice site: 3´ ss) is a fundamental step in precursor messenger RNA (pre-mRNA) splicing. Generally, the U2 small nuclear ribonucleoprotein (snRNP) auxiliary factor (U2AF) heterodimer recognizes the 3´ ss, of which U2AF35 has a dual function: (i) It binds to the intron–exon border of some 3´ ss and (ii) mediates enhancer-binding splicing activators’ interactions with the spliceosome. Alternative mechanisms for 3´ ss recognition have been suggested, yet they are still not thoroughly understood. Here, we analyzed 3´ ss recognition where the intron–exon border is bound by a ubiquitous splicing regulator SRSF1. Using the minigene analysis of two model exons and their mutants, BRCA2 exon 12 and VARS2 exon 17, we showed that the exon inclusion correlated much better with the predicted SRSF1 affinity than 3´ ss quality, which were assessed using the Catalog of Inferred Sequence Binding Preferences of RNA binding proteins (CISBP-RNA) database and maximum entropy algorithm (MaxEnt) predictor and the U2AF35 consensus matrix, respectively. RNA affinity purification proved SRSF1 binding to the model 3´ ss. On the other hand, knockdown experiments revealed that U2AF35 also plays a role in these exons’ inclusion. Most probably, both factors stochastically bind the 3´ ss, supporting exon recognition, more apparently in VARS2 exon 17. Identifying splicing activators as 3´ ss recognition factors is crucial for both a basic understanding of splicing regulation and human genetic diagnostics when assessing variants’ effects on splicing.

Název v anglickém jazyce

Splicing Enhancers at Intron–Exon Borders Participate in Acceptor Splice Sites Recognition

Popis výsledku anglicky

Acceptor splice site recognition (3´ splice site: 3´ ss) is a fundamental step in precursor messenger RNA (pre-mRNA) splicing. Generally, the U2 small nuclear ribonucleoprotein (snRNP) auxiliary factor (U2AF) heterodimer recognizes the 3´ ss, of which U2AF35 has a dual function: (i) It binds to the intron–exon border of some 3´ ss and (ii) mediates enhancer-binding splicing activators’ interactions with the spliceosome. Alternative mechanisms for 3´ ss recognition have been suggested, yet they are still not thoroughly understood. Here, we analyzed 3´ ss recognition where the intron–exon border is bound by a ubiquitous splicing regulator SRSF1. Using the minigene analysis of two model exons and their mutants, BRCA2 exon 12 and VARS2 exon 17, we showed that the exon inclusion correlated much better with the predicted SRSF1 affinity than 3´ ss quality, which were assessed using the Catalog of Inferred Sequence Binding Preferences of RNA binding proteins (CISBP-RNA) database and maximum entropy algorithm (MaxEnt) predictor and the U2AF35 consensus matrix, respectively. RNA affinity purification proved SRSF1 binding to the model 3´ ss. On the other hand, knockdown experiments revealed that U2AF35 also plays a role in these exons’ inclusion. Most probably, both factors stochastically bind the 3´ ss, supporting exon recognition, more apparently in VARS2 exon 17. Identifying splicing activators as 3´ ss recognition factors is crucial for both a basic understanding of splicing regulation and human genetic diagnostics when assessing variants’ effects on splicing.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/NU20-02-00261" target="_blank" >NU20-02-00261: Mechanismy účinku genetických variant LDL receptoru a role genetických variant lipoproteinu(a) při vzniku hypercholesterolémie u pacientu s FH</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Molecular Sciences

ISSN

1422-0067

e-ISSN

—

Svazek periodika

21

Číslo periodika v rámci svazku

18

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

18

Strana od-do

6553

Kód UT WoS článku

000581229300001

EID výsledku v databázi Scopus

2-s2.0-85090660569