Impact of acceptor splice site NAGTAG motif on exon recognition

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209775%3A_____%2F19%3AN0000013" target="_blank" >RIV/00209775:_____/19:N0000013 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14110/19:00108505

Výsledek na webu

<a href="https://link.springer.com/article/10.1007%2Fs11033-019-04734-6" target="_blank" >https://link.springer.com/article/10.1007%2Fs11033-019-04734-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11033-019-04734-6" target="_blank" >10.1007/s11033-019-04734-6</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Impact of acceptor splice site NAGTAG motif on exon recognition

Popis výsledku v původním jazyce

Pre-mRNA splicing is an essential step in gene expression, when introns are removed and exons joined by the complex of proteins called spliceosome. Correct splicing requires a precise exon/intron junction definition, which is determined by a consensual donor and acceptor splice site at the 5′ and 3′ end, respectively. An acceptor splice site (3′ss) consists of highly conserved AG nucleotides in positions E−2 and E−1. These nucleotides can appear in tandem, located 3 bp from each other. Then they are referred to as NAGNAG or tandem 3′ss, which can be alternatively spliced. NAG/TAG 3′ss motif abundance is extremely low and cannot be easily explained by just a nucleotide preference in this position. We tested artificial NAG/TAG motif’s potential negative effect on exon recognition using a minigene assay. Introducing the NAG/TAG motif into seven different exons revealed no general negative effect on exon recognition. The only observed effect was the partial use of the newly formed distal 3′ss. We can conclude that this motif’s extremely low preference in a natural 3′ss is not a consequence of the NAG/TAG motif’s negative effect on exon recognition, but more likely the result of other RNA processing aspects, such as an alternative 3′ss choice, decreased 3′ss strength, or incorporating an amber stop codon.

Název v anglickém jazyce

Impact of acceptor splice site NAGTAG motif on exon recognition

Popis výsledku anglicky

Pre-mRNA splicing is an essential step in gene expression, when introns are removed and exons joined by the complex of proteins called spliceosome. Correct splicing requires a precise exon/intron junction definition, which is determined by a consensual donor and acceptor splice site at the 5′ and 3′ end, respectively. An acceptor splice site (3′ss) consists of highly conserved AG nucleotides in positions E−2 and E−1. These nucleotides can appear in tandem, located 3 bp from each other. Then they are referred to as NAGNAG or tandem 3′ss, which can be alternatively spliced. NAG/TAG 3′ss motif abundance is extremely low and cannot be easily explained by just a nucleotide preference in this position. We tested artificial NAG/TAG motif’s potential negative effect on exon recognition using a minigene assay. Introducing the NAG/TAG motif into seven different exons revealed no general negative effect on exon recognition. The only observed effect was the partial use of the newly formed distal 3′ss. We can conclude that this motif’s extremely low preference in a natural 3′ss is not a consequence of the NAG/TAG motif’s negative effect on exon recognition, but more likely the result of other RNA processing aspects, such as an alternative 3′ss choice, decreased 3′ss strength, or incorporating an amber stop codon.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/NV16-34414A" target="_blank" >NV16-34414A: Určení genových oblastí náchylných ke vzniku mutací ovlivňujících sestřih mRNA</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular Biology Reports

ISSN

0301-4851

e-ISSN

—

Svazek periodika

46

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

2877-2884

Kód UT WoS článku

000470332600028

EID výsledku v databázi Scopus

2-s2.0-85062700137