Nucleotides in both donor and acceptor splice sites are responsible for choice in NAGNAG tandem splice sites

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209775%3A_____%2F21%3AN0000012" target="_blank" >RIV/00209775:_____/21:N0000012 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14110/21:00123981

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s00018-021-03943-2" target="_blank" >https://link.springer.com/article/10.1007/s00018-021-03943-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00018-021-03943-2" target="_blank" >10.1007/s00018-021-03943-2</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Nucleotides in both donor and acceptor splice sites are responsible for choice in NAGNAG tandem splice sites

Popis výsledku v původním jazyce

Among alternative splicing events in the human transcriptome, tandem NAGNAG acceptor splice sites represent an appreciable proportion. Both proximal and distal NAG can be used to produce two splicing isoforms differing by three nucleotides. In some cases, the upstream exon can be alternatively spliced as well, which further increases the number of possible transcripts. In this study, we showed that NAG choice in tandem splice site depends considerably not only on the concerned acceptor, but also on the upstream donor splice site sequence. Using an extensive set of experiments with systematically modified two-exonic minigene systems of AFAP1L2 or CSTD gene, we recognized the third and fifth intronic upstream donor splice site position and the tandem acceptor splice site region spanning from −10 to +2, including NAGNAG itself, as the main drivers. In addition, competition between different branch points and their composition were also shown to play a significant role in NAG choice. All these nucleotide effects appeared almost additive, which explained the high variability in proximal versus distal NAG usage.

Název v anglickém jazyce

Nucleotides in both donor and acceptor splice sites are responsible for choice in NAGNAG tandem splice sites

Popis výsledku anglicky

Among alternative splicing events in the human transcriptome, tandem NAGNAG acceptor splice sites represent an appreciable proportion. Both proximal and distal NAG can be used to produce two splicing isoforms differing by three nucleotides. In some cases, the upstream exon can be alternatively spliced as well, which further increases the number of possible transcripts. In this study, we showed that NAG choice in tandem splice site depends considerably not only on the concerned acceptor, but also on the upstream donor splice site sequence. Using an extensive set of experiments with systematically modified two-exonic minigene systems of AFAP1L2 or CSTD gene, we recognized the third and fifth intronic upstream donor splice site position and the tandem acceptor splice site region spanning from −10 to +2, including NAGNAG itself, as the main drivers. In addition, competition between different branch points and their composition were also shown to play a significant role in NAG choice. All these nucleotide effects appeared almost additive, which explained the high variability in proximal versus distal NAG usage.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

—

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cellular and Molecular Life science

ISSN

1420-682X

e-ISSN

—

Svazek periodika

78

Číslo periodika v rámci svazku

21-22

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

15

Strana od-do

6979-6993

Kód UT WoS článku

000702592500001

EID výsledku v databázi Scopus

2-s2.0-85116039158