Splicing analysis of STAT3 tandem donor suggests non-canonical binding registers for U1 and U6 snRNAs
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F24%3A00079593" target="_blank" >RIV/65269705:_____/24:00079593 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00216224:14110/24:00136122 RIV/00209775:_____/24:N0000016
Výsledek na webu
<a href="https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkae147/7617147?login=true" target="_blank" >https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkae147/7617147?login=true</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/nar/gkae147" target="_blank" >10.1093/nar/gkae147</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Splicing analysis of STAT3 tandem donor suggests non-canonical binding registers for U1 and U6 snRNAs
Popis výsledku v původním jazyce
Tandem donor splice sites (5 ' ss) are unique regions with at least two GU dinucleotides serving as splicing cleavage sites. The Delta 3 tandem 5 ' ss are a specific subclass of 5 ' ss separated by 3 nucleotides which can affect protein function by inserting/deleting a single amino acid. One 5 ' ss is typically preferred, yet factors governing particular 5 ' ss choice are not fully understood. A highly conserved exon 21 of the STAT3 gene was chosen as a model to study Delta 3 tandem 5 ' ss splicing mechanisms. Based on multiple lines of experimental evidence, endogenous U1 snRNA most likely binds only to the upstream 5 ' ss. However, the downstream 5 ' ss is used preferentially, and the splice site choice is not dependent on the exact U1 snRNA binding position. Downstream 5 ' ss usage was sensitive to exact nucleotide composition and dependent on the presence of downstream regulatory region. The downstream 5 ' ss usage could be best explained by two novel interactions with endogenous U6 snRNA. U6 snRNA enables the downstream 5 ' ss usage in STAT3 exon 21 by two mechanisms: (i) binding in a novel non-canonical register and (ii) establishing extended Watson-Crick base pairing with the downstream regulatory region. This study suggests that U6:5 ' ss interaction is more flexible than previously thought. Graphical Abstract
Název v anglickém jazyce
Splicing analysis of STAT3 tandem donor suggests non-canonical binding registers for U1 and U6 snRNAs
Popis výsledku anglicky
Tandem donor splice sites (5 ' ss) are unique regions with at least two GU dinucleotides serving as splicing cleavage sites. The Delta 3 tandem 5 ' ss are a specific subclass of 5 ' ss separated by 3 nucleotides which can affect protein function by inserting/deleting a single amino acid. One 5 ' ss is typically preferred, yet factors governing particular 5 ' ss choice are not fully understood. A highly conserved exon 21 of the STAT3 gene was chosen as a model to study Delta 3 tandem 5 ' ss splicing mechanisms. Based on multiple lines of experimental evidence, endogenous U1 snRNA most likely binds only to the upstream 5 ' ss. However, the downstream 5 ' ss is used preferentially, and the splice site choice is not dependent on the exact U1 snRNA binding position. Downstream 5 ' ss usage was sensitive to exact nucleotide composition and dependent on the presence of downstream regulatory region. The downstream 5 ' ss usage could be best explained by two novel interactions with endogenous U6 snRNA. U6 snRNA enables the downstream 5 ' ss usage in STAT3 exon 21 by two mechanisms: (i) binding in a novel non-canonical register and (ii) establishing extended Watson-Crick base pairing with the downstream regulatory region. This study suggests that U6:5 ' ss interaction is more flexible than previously thought. Graphical Abstract
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10608 - Biochemistry and molecular biology
Návaznosti výsledku
Projekt
<a href="/cs/project/LM2018132" target="_blank" >LM2018132: Národní centrum lékařské genomiky</a><br>
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2024
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Nucleic Acids Research
ISSN
0305-1048
e-ISSN
1362-4962
Svazek periodika
52
Číslo periodika v rámci svazku
10
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
16
Strana od-do
5959-5974
Kód UT WoS článku
001176086700001
EID výsledku v databázi Scopus
2-s2.0-85195778811