Supercoil-Selective binding of protein p53: influence of the protein redox state and roles of p53 domains

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F00%3A00000102" target="_blank" >RIV/00209805:_____/00:00000102 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Supercoil-Selective binding of protein p53: influence of the protein redox state and roles of p53 domains

Popis výsledku v původním jazyce

Full length wild type tumor suppressor protein p53 preferentially binds to supercoiled DNA regardless of the presence or absence of the p53 consensus sequence. This supercoil-selective binding was partially inhibited by the protein oxidation by diamide and p53 sequence-specific DNA binding was completely abolished. The roles of the two protein domains in the SCS binding of reduced (p53red) and oxidized (p53ox) p53 were studied using truncated p53 molecules and monoclonal antibodies (MAb). The primary site responsible for the strong SCS p53 DNA binding is the protein C-terminus. Those p53 constructs lacking free C-terminus in the reduced state bind sc and linear DNAs with similar affinities.

Název v anglickém jazyce

Supercoil-Selective binding of protein p53: influence of the protein redox state and roles of p53 domains

Popis výsledku anglicky

Full length wild type tumor suppressor protein p53 preferentially binds to supercoiled DNA regardless of the presence or absence of the p53 consensus sequence. This supercoil-selective binding was partially inhibited by the protein oxidation by diamide and p53 sequence-specific DNA binding was completely abolished. The roles of the two protein domains in the SCS binding of reduced (p53red) and oxidized (p53ox) p53 were studied using truncated p53 molecules and monoclonal antibodies (MAb). The primary site responsible for the strong SCS p53 DNA binding is the protein C-terminus. Those p53 constructs lacking free C-terminus in the reduced state bind sc and linear DNAs with similar affinities.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2000

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Biomolecular Structure and Dynamics

ISSN

0739-1102

e-ISSN

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Svazek periodika

17

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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