Anterior Gradient-3: a novel biomarker for ovarian cancer that mediates cisplatin resistance in xenograft models

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F12%3A%230000288" target="_blank" >RIV/00209805:_____/12:#0000288 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S002217591200035X" target="_blank" >http://www.sciencedirect.com/science/article/pii/S002217591200035X</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jim.2012.01.013" target="_blank" >10.1016/j.jim.2012.01.013</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Anterior Gradient-3: a novel biomarker for ovarian cancer that mediates cisplatin resistance in xenograft models

Popis výsledku v původním jazyce

The Anterior Gradient genes AGR2 and AGR3 are part of Protein Disulfide Isomerase (PDI) family and harbour core thioredoxin folds (CxxS motifs) that have the potential to regulate protein folding and maturation. Despite the fact that AGR2 and AGR3 genesare contiguous on chromosome 7p21.1-3, AGR3 protein has rarely been identified along with AGR2 protein. Therefore there is little information on how AGR3 protein is expressed in normal and diseased states. A panel of three monoclonal antibodies was generated towards AGR3 protein for identifying novel clinical models that can be used to define whether AGR3 protein could play a positive or negative role in human cancer development. One monoclonal antibody was AGR3-specific and bound a linear epitope thatcould be defined using both pep-scan and phage-peptide library screening. Using this antibody, endogenous AGR3 protein expression was shown to be cytosolic in four human ovarian cancer subtypes; serous, endometrioid, clear cell, and mucin

Název v anglickém jazyce

Anterior Gradient-3: a novel biomarker for ovarian cancer that mediates cisplatin resistance in xenograft models

Popis výsledku anglicky

The Anterior Gradient genes AGR2 and AGR3 are part of Protein Disulfide Isomerase (PDI) family and harbour core thioredoxin folds (CxxS motifs) that have the potential to regulate protein folding and maturation. Despite the fact that AGR2 and AGR3 genesare contiguous on chromosome 7p21.1-3, AGR3 protein has rarely been identified along with AGR2 protein. Therefore there is little information on how AGR3 protein is expressed in normal and diseased states. A panel of three monoclonal antibodies was generated towards AGR3 protein for identifying novel clinical models that can be used to define whether AGR3 protein could play a positive or negative role in human cancer development. One monoclonal antibody was AGR3-specific and bound a linear epitope thatcould be defined using both pep-scan and phage-peptide library screening. Using this antibody, endogenous AGR3 protein expression was shown to be cytosolic in four human ovarian cancer subtypes; serous, endometrioid, clear cell, and mucin

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of immunological methods

ISSN

0022-1759

e-ISSN

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Svazek periodika

378

Číslo periodika v rámci svazku

1-2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

13

Strana od-do

20-32

Kód UT WoS článku

000304285900003

EID výsledku v databázi Scopus

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