Magnetic bead-based hybridization assay for electrochemical detection of microRNAs

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F14%3A%230000503" target="_blank" >RIV/00209805:_____/14:#0000503 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.aca.2014.01.023" target="_blank" >http://dx.doi.org/10.1016/j.aca.2014.01.023</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aca.2014.01.023" target="_blank" >10.1016/j.aca.2014.01.023</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Magnetic bead-based hybridization assay for electrochemical detection of microRNAs

Popis výsledku v původním jazyce

Aberrant expression of microRNAs (miRNAs), short non-coding RNA molecules regulating gene expression, is often found in tumor cells, making the miRNAs suitable candidates as cancer biomarkers. Electrochemistry is an interesting alternative to current standard methods of miRNA detection by offering cheaper instrumentation and faster assays times. In this paper, we labeled miRNA in a quick, simple, two-step procedure with electroactive complex of osmium(VI) and 2,2´-bipyridine, Os(VI)bipy, which specifically binds to the ribose at the 3´-end of the miRNA, and hybridized such labeled miRNA with biotinylated capture probe attached to the streptavidin magnetic beads. Labeled miRNA was then detected at hanging mercury drop electrode at femtomole level due toan electrocatalytic nature of the peak from the Os(VI)bipy label. We obtained good selectivity of the assay using elevated hybridization temperatures for better discrimination of perfect duplex from single and double mismatches. After op

Název v anglickém jazyce

Magnetic bead-based hybridization assay for electrochemical detection of microRNAs

Popis výsledku anglicky

Aberrant expression of microRNAs (miRNAs), short non-coding RNA molecules regulating gene expression, is often found in tumor cells, making the miRNAs suitable candidates as cancer biomarkers. Electrochemistry is an interesting alternative to current standard methods of miRNA detection by offering cheaper instrumentation and faster assays times. In this paper, we labeled miRNA in a quick, simple, two-step procedure with electroactive complex of osmium(VI) and 2,2´-bipyridine, Os(VI)bipy, which specifically binds to the ribose at the 3´-end of the miRNA, and hybridized such labeled miRNA with biotinylated capture probe attached to the streptavidin magnetic beads. Labeled miRNA was then detected at hanging mercury drop electrode at femtomole level due toan electrocatalytic nature of the peak from the Os(VI)bipy label. We obtained good selectivity of the assay using elevated hybridization temperatures for better discrimination of perfect duplex from single and double mismatches. After op

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CG - Elektrochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytica chimica acta

ISSN

0003-2670

e-ISSN

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Svazek periodika

813

Číslo periodika v rámci svazku

27 February

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

35-40

Kód UT WoS článku

000331556000004

EID výsledku v databázi Scopus

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