Os(VI)bipy-based electrochemical assay for detection of specific microRNAs as potential cancer biomarkers

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F13%3A00422262" target="_blank" >RIV/68081707:_____/13:00422262 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00209805:_____/13:#0000415

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.elecom.2013.04.009" target="_blank" >http://dx.doi.org/10.1016/j.elecom.2013.04.009</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.elecom.2013.04.009" target="_blank" >10.1016/j.elecom.2013.04.009</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Os(VI)bipy-based electrochemical assay for detection of specific microRNAs as potential cancer biomarkers

Popis výsledku v původním jazyce

Recently, altered expression levels of microRNAs (miRNAs) - short noncoding RNA molecules which bind to mRNAs and thus regulate gene expression - were observed in many cancer cells. miRNA expression profiling is therefore of great interest, but current standard methods are still considered relatively laborious and expensive. Electrochemistry has a potential to become quick and inexpensive alternative. Here, we describe modification of miRNA with an electroactive complex composed of six-valent osmium and2,2'-bipyridine, Os(VI)bipy, specifically binding to the 3'-end of the ribose, which is detectable at hanging mercury drop electrode at femtomole level due to an electrocatalytic nature of a resulting signal. By combining miRNA labeling step with magnetic beads-based hybridization assay, detection of specific miRNA sequence from a mixture of other noncomplementary miRNAs was possible. (c) 2013 Elsevier B.V. All rights reserved.

Název v anglickém jazyce

Os(VI)bipy-based electrochemical assay for detection of specific microRNAs as potential cancer biomarkers

Popis výsledku anglicky

Recently, altered expression levels of microRNAs (miRNAs) - short noncoding RNA molecules which bind to mRNAs and thus regulate gene expression - were observed in many cancer cells. miRNA expression profiling is therefore of great interest, but current standard methods are still considered relatively laborious and expensive. Electrochemistry has a potential to become quick and inexpensive alternative. Here, we describe modification of miRNA with an electroactive complex composed of six-valent osmium and2,2'-bipyridine, Os(VI)bipy, specifically binding to the 3'-end of the ribose, which is detectable at hanging mercury drop electrode at femtomole level due to an electrocatalytic nature of a resulting signal. By combining miRNA labeling step with magnetic beads-based hybridization assay, detection of specific miRNA sequence from a mixture of other noncomplementary miRNAs was possible. (c) 2013 Elsevier B.V. All rights reserved.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrochemistry Communications

ISSN

1388-2481

e-ISSN

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Svazek periodika

33

Číslo periodika v rámci svazku

AUG2013

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

4

Strana od-do

55-58

Kód UT WoS článku

000323024600014

EID výsledku v databázi Scopus

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