Novel insights into the assembly and function of human nuclear-encoded cytochrome c oxidase subunits 4, 5a, 6a, 7a and 7b

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F10%3A6854" target="_blank" >RIV/00216208:11110/10:6854 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00064165:_____/10:6854

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Novel insights into the assembly and function of human nuclear-encoded cytochrome c oxidase subunits 4, 5a, 6a, 7a and 7b

Popis výsledku v původním jazyce

Mammalian CcO (cytochrome c oxidase) is a complex composed of 13 different structural subunits encoded by both the mitochondrial and nuclear genomes. Knockdown of Cox4, Cox5a and Cox6a resulted in reduced CcO activity, diminished affinity of the residualenzyme for oxygen, decreased holoCcO and CcO dimer levels, increased accumulation of CcO subcomplexes and an altered pattern of respiratory supercomplexes. An analysis of the patterns of CcO subcomplexes found in both knockdowns and cells overexpressingepitope-tagged Cox6a, Cox7a and Cox7b identified a novel CcO assembly intermediate, identified the entry points of three late-assembled subunits and demonstrated the interdependence of Cox4 and Cox5a assembly. The ectopic expression of the heart/muscle-specific isoform of the Cox6 subunit (COX6A2) resulted in restoration of both CcO holoenzyme and activity in COX6A1-knockdown cells contrary to the unaltered levels of COX6A2 mRNA in these cells, suggesting the existence of a fixed expres

Název v anglickém jazyce

Novel insights into the assembly and function of human nuclear-encoded cytochrome c oxidase subunits 4, 5a, 6a, 7a and 7b

Popis výsledku anglicky

Mammalian CcO (cytochrome c oxidase) is a complex composed of 13 different structural subunits encoded by both the mitochondrial and nuclear genomes. Knockdown of Cox4, Cox5a and Cox6a resulted in reduced CcO activity, diminished affinity of the residualenzyme for oxygen, decreased holoCcO and CcO dimer levels, increased accumulation of CcO subcomplexes and an altered pattern of respiratory supercomplexes. An analysis of the patterns of CcO subcomplexes found in both knockdowns and cells overexpressingepitope-tagged Cox6a, Cox7a and Cox7b identified a novel CcO assembly intermediate, identified the entry points of three late-assembled subunits and demonstrated the interdependence of Cox4 and Cox5a assembly. The ectopic expression of the heart/muscle-specific isoform of the Cox6 subunit (COX6A2) resulted in restoration of both CcO holoenzyme and activity in COX6A1-knockdown cells contrary to the unaltered levels of COX6A2 mRNA in these cells, suggesting the existence of a fixed expres

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biochemical Journal

ISSN

0264-6021

e-ISSN

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Svazek periodika

428

Číslo periodika v rámci svazku

part 3

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

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Kód UT WoS článku

000278914400005

EID výsledku v databázi Scopus

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