Placental tissue as model for pilot study focused on RNA analysis from human foetal tissue

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F11%3A10066" target="_blank" >RIV/00216208:11110/11:10066 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00064165:_____/11:10066

Výsledek na webu

<a href="http://pmr.cuni.cz/file/5587/pmr2011a0010.pdf" target="_blank" >http://pmr.cuni.cz/file/5587/pmr2011a0010.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Placental tissue as model for pilot study focused on RNA analysis from human foetal tissue

Popis výsledku v původním jazyce

Early neonatal adaptation to extrauterine life is i.a. dependent on effective mitochondrial biogenesis during foetal development. Understanding of mitochondrial biogenesis is limited, because only scarce data are available from prenatal studies includingRNA analyses in human foetal tissues. Aims of the study were focused on the factors affecting RNA quality in human placental tissue (HPT) including temperature, time period before HPT freezing and the Apgar score. In addition, optimal reference genes were studied by analysing six candidate genes (ATP5O, SDHA, TBP, HPRT, PMBS, ATP6) for mRNA quantification by qPCR in HPT. Samples of HPT were obtained after the birth of 20 term neonates. Then RNA integrity, purity and yield were evaluated in dependence on the factors.The results showed that human placenta represents easily obtainable source of foetal tissue for studies concerning mitochondrial biogenesis. We demonstrated that the critical limit for optimal storage and handling of HPT are

Název v anglickém jazyce

Placental tissue as model for pilot study focused on RNA analysis from human foetal tissue

Popis výsledku anglicky

Early neonatal adaptation to extrauterine life is i.a. dependent on effective mitochondrial biogenesis during foetal development. Understanding of mitochondrial biogenesis is limited, because only scarce data are available from prenatal studies includingRNA analyses in human foetal tissues. Aims of the study were focused on the factors affecting RNA quality in human placental tissue (HPT) including temperature, time period before HPT freezing and the Apgar score. In addition, optimal reference genes were studied by analysing six candidate genes (ATP5O, SDHA, TBP, HPRT, PMBS, ATP6) for mRNA quantification by qPCR in HPT. Samples of HPT were obtained after the birth of 20 term neonates. Then RNA integrity, purity and yield were evaluated in dependence on the factors.The results showed that human placenta represents easily obtainable source of foetal tissue for studies concerning mitochondrial biogenesis. We demonstrated that the critical limit for optimal storage and handling of HPT are

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FG - Pediatrie

OECD FORD obor

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Projekt

<a href="/cs/project/NS10581" target="_blank" >NS10581: Genetická podstata poruch cytochrom c oxidázy</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Prague Medical Report

ISSN

1214-6994

e-ISSN

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Svazek periodika

112

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

9

Strana od-do

93-101

Kód UT WoS článku

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EID výsledku v databázi Scopus

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