Apoptosis and necrosis in vaccinia virus-infected He La G and BSC-40 cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F11%3A8927" target="_blank" >RIV/00216208:11110/11:8927 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.virusres.2011.05.005" target="_blank" >http://dx.doi.org/10.1016/j.virusres.2011.05.005</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Apoptosis and necrosis in vaccinia virus-infected He La G and BSC-40 cells

Popis výsledku v původním jazyce

In most cells, vaccinia virus (VACV) infection is considered to cause a lytic cell death, an equivalent of necrosis. In this paper, we have further analyzed the type of cell death in VACV-infected cells HeLa G and BSC-40. In a cell-based flow cytometricassay, we showed a specific activation of caspase-2 and 4 in HeLa G and BSC-40 cells infected with VACV, strain WR. The actual activity of the two caspases, but also of caspase-3, was then confirmed in lysates of infected HeLa G, but not in BSC-40 cells.PARP cleavage, as well as morphological features of apoptosis and/or activity of the executioner caspase-3 in situ were found increased only in infected HeLa G cells. Finally, vaccination strains Dryvax and Praha were found to induce apoptosis in both HeLa G and BSC-40 cells, as characterized morphologically and by PARP cleavage. These findings may be important for understanding the differences in VACV-host interactions and post-vaccination complications in different individuals.

Název v anglickém jazyce

Apoptosis and necrosis in vaccinia virus-infected He La G and BSC-40 cells

Popis výsledku anglicky

In most cells, vaccinia virus (VACV) infection is considered to cause a lytic cell death, an equivalent of necrosis. In this paper, we have further analyzed the type of cell death in VACV-infected cells HeLa G and BSC-40. In a cell-based flow cytometricassay, we showed a specific activation of caspase-2 and 4 in HeLa G and BSC-40 cells infected with VACV, strain WR. The actual activity of the two caspases, but also of caspase-3, was then confirmed in lysates of infected HeLa G, but not in BSC-40 cells.PARP cleavage, as well as morphological features of apoptosis and/or activity of the executioner caspase-3 in situ were found increased only in infected HeLa G cells. Finally, vaccination strains Dryvax and Praha were found to induce apoptosis in both HeLa G and BSC-40 cells, as characterized morphologically and by PARP cleavage. These findings may be important for understanding the differences in VACV-host interactions and post-vaccination complications in different individuals.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Virus Research

ISSN

0168-1702

e-ISSN

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Svazek periodika

160

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

40-50

Kód UT WoS článku

000295345600005

EID výsledku v databázi Scopus

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