Caspase-2 is involved in cell death induction by taxanes in breast cancer cells
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F13%3A43907485" target="_blank" >RIV/00216208:11120/13:43907485 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1186/1475-2867-13-42" target="_blank" >http://dx.doi.org/10.1186/1475-2867-13-42</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/1475-2867-13-42" target="_blank" >10.1186/1475-2867-13-42</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Caspase-2 is involved in cell death induction by taxanes in breast cancer cells
Popis výsledku v původním jazyce
We studied the role of caspase-2 in apoptosis induction by taxanes (paclitaxel, novel taxane SB-T-1216) in breast cancer cells using SK-BR-3 and MCF-7 cell lines. Both taxanes induced apoptosis in SK-BR-3 as well as MCF-7 cells. Caspase-2 activity in SK-BR-3 cells increased approximately 15-fold within 48 h after the application of both taxanes at the death-inducing concentration (100 nM). In MCF-7 cells, caspase-2 activity increased approximately 11-fold within 60 h after the application of taxanes (300 nM). Caspase-2 activation was confirmed by decreasing levels of procaspase-2, increasing levels of cleaved caspase-2 and the cleavage of caspase-2 substrate golgin-160. The inhibition of caspase-2 expression using siRNA increased the number of surviving cells more than 2-fold in MCF-7 cells, and at least 4-fold in SK-BR-3 cells, 96 h after the application of death-inducing concentration of taxanes. The inhibition of caspase-2 expression also resulted in decreased cleavage of initiator
Název v anglickém jazyce
Caspase-2 is involved in cell death induction by taxanes in breast cancer cells
Popis výsledku anglicky
We studied the role of caspase-2 in apoptosis induction by taxanes (paclitaxel, novel taxane SB-T-1216) in breast cancer cells using SK-BR-3 and MCF-7 cell lines. Both taxanes induced apoptosis in SK-BR-3 as well as MCF-7 cells. Caspase-2 activity in SK-BR-3 cells increased approximately 15-fold within 48 h after the application of both taxanes at the death-inducing concentration (100 nM). In MCF-7 cells, caspase-2 activity increased approximately 11-fold within 60 h after the application of taxanes (300 nM). Caspase-2 activation was confirmed by decreasing levels of procaspase-2, increasing levels of cleaved caspase-2 and the cleavage of caspase-2 substrate golgin-160. The inhibition of caspase-2 expression using siRNA increased the number of surviving cells more than 2-fold in MCF-7 cells, and at least 4-fold in SK-BR-3 cells, 96 h after the application of death-inducing concentration of taxanes. The inhibition of caspase-2 expression also resulted in decreased cleavage of initiator
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
FD - Onkologie a hematologie
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/GA301%2F09%2F0362" target="_blank" >GA301/09/0362: Úloha kaspázy 2 v indukci apoptózy taxany u buněk nádorů prsu</a><br>
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2013
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Cancer Cell International
ISSN
1475-2867
e-ISSN
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Svazek periodika
13
Číslo periodika v rámci svazku
42
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
15
Strana od-do
1-15
Kód UT WoS článku
000320553000001
EID výsledku v databázi Scopus
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