The role of individual caspases in cell death induction by taxanes in breast cancer cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F15%3A43909312" target="_blank" >RIV/00216208:11120/15:43909312 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1186/s12935-015-0155-7" target="_blank" >http://dx.doi.org/10.1186/s12935-015-0155-7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12935-015-0155-7" target="_blank" >10.1186/s12935-015-0155-7</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The role of individual caspases in cell death induction by taxanes in breast cancer cells

Popis výsledku v původním jazyce

Background: In previous study we showed that caspase-2 plays the role of an apical caspase in cell death induction by taxanes in breast cancer cells. This study deals with the role of other caspases. We tested breast cancer cell lines SK-BR-3 (functionalcaspase-3) and MCF-7 (nonfunctional caspase-3). Methods and Results: Using western blot analysis we demonstrated the activation of initiator caspase-8 and -9 as well as executioner caspase-6 and -7 in both tested cell lines after application of taxanes(paclitaxel, SB-T-1216) at death-inducing concentrations. Caspase-3 activation was also found in SK-BR-3 cells. Employing specific siRNAs after taxane application, suppression of caspase-3 expression significantly increased the number of surviving SK-BR-3 cells. Inhibition of caspase-7 expression also increased the number of surviving SK-BR-3 and MCF-7 cells. On the other hand, suppression of caspase-8 and caspase-9 expression had no significant effect on cell survival. However, caspase-

Název v anglickém jazyce

The role of individual caspases in cell death induction by taxanes in breast cancer cells

Popis výsledku anglicky

Background: In previous study we showed that caspase-2 plays the role of an apical caspase in cell death induction by taxanes in breast cancer cells. This study deals with the role of other caspases. We tested breast cancer cell lines SK-BR-3 (functionalcaspase-3) and MCF-7 (nonfunctional caspase-3). Methods and Results: Using western blot analysis we demonstrated the activation of initiator caspase-8 and -9 as well as executioner caspase-6 and -7 in both tested cell lines after application of taxanes(paclitaxel, SB-T-1216) at death-inducing concentrations. Caspase-3 activation was also found in SK-BR-3 cells. Employing specific siRNAs after taxane application, suppression of caspase-3 expression significantly increased the number of surviving SK-BR-3 cells. Inhibition of caspase-7 expression also increased the number of surviving SK-BR-3 and MCF-7 cells. On the other hand, suppression of caspase-8 and caspase-9 expression had no significant effect on cell survival. However, caspase-

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA301%2F09%2F0362" target="_blank" >GA301/09/0362: Úloha kaspázy 2 v indukci apoptózy taxany u buněk nádorů prsu</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cancer Cell International

ISSN

1475-2867

e-ISSN

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Svazek periodika

15

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

16

Strana od-do

"Article ID 8; 1"-16

Kód UT WoS článku

000350172000001

EID výsledku v databázi Scopus

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