The evaluation of survival and proliferation of lymphocytes in autologous mixed leukocyte reaction with dendritic cells. The comparison of incorporation of (3)H-thymidine and differential gating method

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F11%3A9189" target="_blank" >RIV/00216208:11110/11:9189 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00023736:_____/11:00009277 RIV/00064165:_____/11:9189

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.cellimm.2011.06.006" target="_blank" >http://dx.doi.org/10.1016/j.cellimm.2011.06.006</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The evaluation of survival and proliferation of lymphocytes in autologous mixed leukocyte reaction with dendritic cells. The comparison of incorporation of (3)H-thymidine and differential gating method

Popis výsledku v původním jazyce

Dendritic cells (DCs) play the key role in T-lymphocyte proliferation and induction of antitumour response. The mixed leukocyte reaction (MLR) of T-lymphocytes and DCs is essential instrument for immunological mechanisms studies. Conventionally used method for determination of T-lymphocytes proliferation, 3H-thymidine incorporation, provides only general information. The method of flow cytometry and differential gating, based on time limited acquisition of events and on its distribution according to forward and side scatter values, seems to be more suitable for quantitative and qualitative analysis of T-lymphocyte proliferation. We decided to compare these methods and determine mutual correlation and compatibility. Eleven patients were studied. Both methods retained consistency with regard to survival and proliferation of lymphocytes. However, the correlation was not convincing. Therefore, both methods might be used for evaluation of MLR, but each of them gives specific and complementa

Název v anglickém jazyce

The evaluation of survival and proliferation of lymphocytes in autologous mixed leukocyte reaction with dendritic cells. The comparison of incorporation of (3)H-thymidine and differential gating method

Popis výsledku anglicky

Dendritic cells (DCs) play the key role in T-lymphocyte proliferation and induction of antitumour response. The mixed leukocyte reaction (MLR) of T-lymphocytes and DCs is essential instrument for immunological mechanisms studies. Conventionally used method for determination of T-lymphocytes proliferation, 3H-thymidine incorporation, provides only general information. The method of flow cytometry and differential gating, based on time limited acquisition of events and on its distribution according to forward and side scatter values, seems to be more suitable for quantitative and qualitative analysis of T-lymphocyte proliferation. We decided to compare these methods and determine mutual correlation and compatibility. Eleven patients were studied. Both methods retained consistency with regard to survival and proliferation of lymphocytes. However, the correlation was not convincing. Therefore, both methods might be used for evaluation of MLR, but each of them gives specific and complementa

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EC - Imunologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cellular Immunology

ISSN

0008-8749

e-ISSN

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Svazek periodika

271

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

78-84

Kód UT WoS článku

000297658400012

EID výsledku v databázi Scopus

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