Alterations in methylation status of immune response genes promoters in cell-free DNA during a hemodialysis procedure
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F12%3A11733" target="_blank" >RIV/00216208:11110/12:11733 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00216208:11120/12:43896678 RIV/00064173:_____/12:43896678 RIV/00064165:_____/12:11733
Výsledek na webu
<a href="http://dx.doi.org/10.1517/14712598.2012.676034" target="_blank" >http://dx.doi.org/10.1517/14712598.2012.676034</a>
DOI - Digital Object Identifier
—
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Alterations in methylation status of immune response genes promoters in cell-free DNA during a hemodialysis procedure
Popis výsledku v původním jazyce
Objective: Elevations of cell-free DNA (cfDNA) concentrations during hemodialysis (HD) sessions were reported in numerous studies regardless of an applied therapeutic protocol. It is generally thought that the elevated concentrations represent the consequence of apoptosis on the dialysis membranes. No data concerning the qualitative characteristics of cfDNAs in HD patients have been published till today; therefore, we focus on the promoter methylation status of genes involved in immune response. Research design and methods: We isolated cfDNA from randomly selected patients before and after a HD session and from healthy subjects examined two times per day with 4 h interval. The extent of promoter methylation of 24 genes involved in immune response was examined using the 'EpiTect Methyl qPCR Array Inflammatory Response and Autoimmunity'. Results: In our pilot study, we discovered significant changes in methylation patterns of genes IL-7, IL-13, IL-17C and TYK2 between HD patients and hea
Název v anglickém jazyce
Alterations in methylation status of immune response genes promoters in cell-free DNA during a hemodialysis procedure
Popis výsledku anglicky
Objective: Elevations of cell-free DNA (cfDNA) concentrations during hemodialysis (HD) sessions were reported in numerous studies regardless of an applied therapeutic protocol. It is generally thought that the elevated concentrations represent the consequence of apoptosis on the dialysis membranes. No data concerning the qualitative characteristics of cfDNAs in HD patients have been published till today; therefore, we focus on the promoter methylation status of genes involved in immune response. Research design and methods: We isolated cfDNA from randomly selected patients before and after a HD session and from healthy subjects examined two times per day with 4 h interval. The extent of promoter methylation of 24 genes involved in immune response was examined using the 'EpiTect Methyl qPCR Array Inflammatory Response and Autoimmunity'. Results: In our pilot study, we discovered significant changes in methylation patterns of genes IL-7, IL-13, IL-17C and TYK2 between HD patients and hea
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
—
Návaznosti výsledku
Projekt
—
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2012
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Expert opinion on biological therapy
ISSN
1471-2598
e-ISSN
—
Svazek periodika
12
Číslo periodika v rámci svazku
1
Stát vydavatele periodika
CH - Švýcarská konfederace
Počet stran výsledku
7
Strana od-do
"S27"-"S33"
Kód UT WoS článku
000304405600005
EID výsledku v databázi Scopus
—