Dipeptidyl peptidase-IV inhibits glioma cell growth independent of its enzymatic activity

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F12%3A12122" target="_blank" >RIV/00216208:11110/12:12122 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378050:_____/12:00387877

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.biocel.2012.01.011" target="_blank" >http://dx.doi.org/10.1016/j.biocel.2012.01.011</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Dipeptidyl peptidase-IV inhibits glioma cell growth independent of its enzymatic activity

Popis výsledku v původním jazyce

The report analyzes the expression and function of DPP-IV in glioma cells. Variable expression of DPP-IV and homologous proteases fibroblast activation protein, DPP8 and DPP9 was observed in primary cell cultures derived from high-grade gliomas and the overall DPP-IV-like enzymatic activity was negatively associated with their in vitro growth. The DPP-IV positive subpopulation isolated from the primary cell cultures using immunomagnetic separation exhibited slower proliferation, in addition, transfection of the wild as well as the enzymatically inactive mutant DPP-IV resulted in the reduced growth, migration and adhesion in different glioma cell lines in vitro. It also suppressed glioma growth in an orthotopic xenotransplantation mouse model. Microarray analysis of glioma cells with forced DPP-IV expression revealed differential expression of several candidate genes not linked to the tumor suppressive effects of DPP-IV in previous studies with enrichment of genes associated with cell p

Název v anglickém jazyce

Dipeptidyl peptidase-IV inhibits glioma cell growth independent of its enzymatic activity

Popis výsledku anglicky

The report analyzes the expression and function of DPP-IV in glioma cells. Variable expression of DPP-IV and homologous proteases fibroblast activation protein, DPP8 and DPP9 was observed in primary cell cultures derived from high-grade gliomas and the overall DPP-IV-like enzymatic activity was negatively associated with their in vitro growth. The DPP-IV positive subpopulation isolated from the primary cell cultures using immunomagnetic separation exhibited slower proliferation, in addition, transfection of the wild as well as the enzymatically inactive mutant DPP-IV resulted in the reduced growth, migration and adhesion in different glioma cell lines in vitro. It also suppressed glioma growth in an orthotopic xenotransplantation mouse model. Microarray analysis of glioma cells with forced DPP-IV expression revealed differential expression of several candidate genes not linked to the tumor suppressive effects of DPP-IV in previous studies with enrichment of genes associated with cell p

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Biochemistry & Cell Biology

ISSN

1357-2725

e-ISSN

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Svazek periodika

44

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

738-747

Kód UT WoS článku

000303558600014

EID výsledku v databázi Scopus

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