Human keratinocyte growth and differentiation on acellular porcine dermal matrix in relation to wound healing potential

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F12%3A12275" target="_blank" >RIV/00216208:11110/12:12275 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00064173:_____/12:43901101 RIV/00216208:11120/12:43901101

Výsledek na webu

<a href="http://dx.doi.org/10.1100/2012/727352" target="_blank" >http://dx.doi.org/10.1100/2012/727352</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Human keratinocyte growth and differentiation on acellular porcine dermal matrix in relation to wound healing potential

Popis výsledku v původním jazyce

A number of implantable biomaterials derived from animal tissues are now used in modern surgery. Xe-Derma is a dry, sterile, acellular porcine dermis. It has a remarkable healing effect on burns and other wounds. Our hypothesis was that the natural biological structure of Xe-Derma plays an important role in keratinocyte proliferation and formation of epidermal architecture in vitro as well as in vivo. The bioactivity of Xe-Derma was studied by a cell culture assay. We analyzed growth and differentiationof human keratinocytes cultured in vitro on Xe-Derma, and we compared the results with formation of neoepidermis in the deep dermal wounds treated with Xe-Derma. Keratinocytes cultured on Xe-Derma submerged in the culture medium achieved confluence in 7-10 days. After lifting the cultures to the air-liquid interface, the keratinocytes were stratified and differentiated within one week, forming an epidermis with basal, spinous, granular, and stratum corneum layers. Immunohistochemical de

Název v anglickém jazyce

Human keratinocyte growth and differentiation on acellular porcine dermal matrix in relation to wound healing potential

Popis výsledku anglicky

A number of implantable biomaterials derived from animal tissues are now used in modern surgery. Xe-Derma is a dry, sterile, acellular porcine dermis. It has a remarkable healing effect on burns and other wounds. Our hypothesis was that the natural biological structure of Xe-Derma plays an important role in keratinocyte proliferation and formation of epidermal architecture in vitro as well as in vivo. The bioactivity of Xe-Derma was studied by a cell culture assay. We analyzed growth and differentiationof human keratinocytes cultured in vitro on Xe-Derma, and we compared the results with formation of neoepidermis in the deep dermal wounds treated with Xe-Derma. Keratinocytes cultured on Xe-Derma submerged in the culture medium achieved confluence in 7-10 days. After lifting the cultures to the air-liquid interface, the keratinocytes were stratified and differentiated within one week, forming an epidermis with basal, spinous, granular, and stratum corneum layers. Immunohistochemical de

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FJ - Chirurgie včetně transplantologie

OECD FORD obor

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Projekt

<a href="/cs/project/NS10507" target="_blank" >NS10507: Inovace léčby kriticky popálených pacientů krytím široce síťovaných autotransplantátů lidskými keratinocyty kultivovanými in vitro na acelulární xenomatrix</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

The Scientific World Journal

ISSN

1537-744X

e-ISSN

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Svazek periodika

Neuveden

Číslo periodika v rámci svazku

Article Number 727352

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

1-8

Kód UT WoS článku

000306811600001

EID výsledku v databázi Scopus

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