The effect of different biologic and biosynthetic wound covers on keratinocyte growth, stratification and differentiation in vitro

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F14%3A10282303" target="_blank" >RIV/00216208:11110/14:10282303 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00064211:_____/14:#0000417 RIV/00216208:11120/14:43909161

Výsledek na webu

<a href="http://dx.doi.org/10.1177/2041731414554966" target="_blank" >http://dx.doi.org/10.1177/2041731414554966</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1177/2041731414554966" target="_blank" >10.1177/2041731414554966</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The effect of different biologic and biosynthetic wound covers on keratinocyte growth, stratification and differentiation in vitro

Popis výsledku v původním jazyce

The purpose of this study was to compare, by means of in vitro cultivation technique, five marketed brands of wound covers used in the treatment of burns and other skin defects (Biobrane((R)), Suprathel((R)), Veloderm((R)), Xe-Derma((R)), and Xenoderm((R))) for their ability to stimulate the keratinocyte growth, stratification, and differentiation. In three independent experiments, human keratinocytes were grown on the tested covers in organotypic cultures by the 3T3 feeder layer technique. Vertical paraffin sections of the wound covers with keratinocytes were processed using hematoxylin-eosin staining and immunostaining for involucrin. Keratinocyte populations on the dressings were assessed for (1) number of keratinocyte strata (primary variable), (2)quantitative growth, (3) thickness of the keratinocyte layer, and (4) cell differentiation. The Xe-Derma wound cover provided the best support to keratinocyte proliferation and stratification, with the number of keratinocyte strata signi

Název v anglickém jazyce

The effect of different biologic and biosynthetic wound covers on keratinocyte growth, stratification and differentiation in vitro

Popis výsledku anglicky

The purpose of this study was to compare, by means of in vitro cultivation technique, five marketed brands of wound covers used in the treatment of burns and other skin defects (Biobrane((R)), Suprathel((R)), Veloderm((R)), Xe-Derma((R)), and Xenoderm((R))) for their ability to stimulate the keratinocyte growth, stratification, and differentiation. In three independent experiments, human keratinocytes were grown on the tested covers in organotypic cultures by the 3T3 feeder layer technique. Vertical paraffin sections of the wound covers with keratinocytes were processed using hematoxylin-eosin staining and immunostaining for involucrin. Keratinocyte populations on the dressings were assessed for (1) number of keratinocyte strata (primary variable), (2)quantitative growth, (3) thickness of the keratinocyte layer, and (4) cell differentiation. The Xe-Derma wound cover provided the best support to keratinocyte proliferation and stratification, with the number of keratinocyte strata signi

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FJ - Chirurgie včetně transplantologie

OECD FORD obor

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Projekt

<a href="/cs/project/NT11392" target="_blank" >NT11392: Výzkum použití nového acelulárního biomateriálu biologického původu Xe-Derma pro rekonstrukci břišní stěny</a><br>

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Tissue Engineering

ISSN

2041-7314

e-ISSN

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Svazek periodika

5

Číslo periodika v rámci svazku

Oct 14

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

1-8

Kód UT WoS článku

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EID výsledku v databázi Scopus

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