Microfiltration method of removal of bacterial contaminants and their monitoring by nitric oxide and Limulus assays

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F13%3A10138963" target="_blank" >RIV/00216208:11110/13:10138963 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378041:_____/13:00382480 RIV/61388963:_____/13:00382480 RIV/00216208:11140/13:10138963

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.niox.2012.08.078" target="_blank" >http://dx.doi.org/10.1016/j.niox.2012.08.078</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.niox.2012.08.078" target="_blank" >10.1016/j.niox.2012.08.078</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Microfiltration method of removal of bacterial contaminants and their monitoring by nitric oxide and Limulus assays

Popis výsledku v původním jazyce

Similar to lipopolysaccharide (LPS), a product of Gram-negative bacteria, the signal macromolecules of Gram-positive bacteria lipoteichoic acid (LTA) and peptidoglycan (PGN) possess multiple biological activities. They may be a source of misinterpretation of experimental findings. We have found that not only LPS but also LTA and PGN can be detected by the Limulus amebocyte lysate (LAL) assay. All of them stimulate the high output in vitro nitric oxide (NO) production of in rat peritoneal cells. The onset of the NO enhancement was observed with 25-100 pg/ml of LPS and 25-100 ng/ml of PGN and LTA. Polymyxin B (PMX), if applied at concentration 10,000-fold higher than that of LPS, can completely inhibit the NO and LAL binding responses of LPS. The NO-stimulatory and LAL-binding properties of LTA and PGN are not eliminated by PMX. Handling of LPS contamination with PMX may be associated with serious problems because it possesses intrinsic biological activity and becomes cytotoxic at concen

Název v anglickém jazyce

Microfiltration method of removal of bacterial contaminants and their monitoring by nitric oxide and Limulus assays

Popis výsledku anglicky

Similar to lipopolysaccharide (LPS), a product of Gram-negative bacteria, the signal macromolecules of Gram-positive bacteria lipoteichoic acid (LTA) and peptidoglycan (PGN) possess multiple biological activities. They may be a source of misinterpretation of experimental findings. We have found that not only LPS but also LTA and PGN can be detected by the Limulus amebocyte lysate (LAL) assay. All of them stimulate the high output in vitro nitric oxide (NO) production of in rat peritoneal cells. The onset of the NO enhancement was observed with 25-100 pg/ml of LPS and 25-100 ng/ml of PGN and LTA. Polymyxin B (PMX), if applied at concentration 10,000-fold higher than that of LPS, can completely inhibit the NO and LAL binding responses of LPS. The NO-stimulatory and LAL-binding properties of LTA and PGN are not eliminated by PMX. Handling of LPS contamination with PMX may be associated with serious problems because it possesses intrinsic biological activity and becomes cytotoxic at concen

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FR - Farmakologie a lékárnická chemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nitric Oxide - Biology and Chemistry

ISSN

1089-8603

e-ISSN

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Svazek periodika

28

Číslo periodika v rámci svazku

Jan 15

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

1-7

Kód UT WoS článku

000315065900001

EID výsledku v databázi Scopus

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