Rapid cooling of the amniotic membrane as a model system for the vitrification of posterior corneal lamellae

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F14%3A10285228" target="_blank" >RIV/00216208:11110/14:10285228 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s10561-013-9388-7" target="_blank" >http://dx.doi.org/10.1007/s10561-013-9388-7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10561-013-9388-7" target="_blank" >10.1007/s10561-013-9388-7</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Rapid cooling of the amniotic membrane as a model system for the vitrification of posterior corneal lamellae

Popis výsledku v původním jazyce

To vitrify human amniotic membrane specimens so that the maximum of epithelial cells survives in order to develop a procedure for the eventual vitrification of posterior corneal lamellae without using cryoprotective agents. To assess different methods oftissue sample preparation preceding vitrification. In group 1, the amniotic membrane specimens were stretched on nitrocellulose support. In group 2, mechanical pressure was used to remove the excess culture medium between the support and the membrane. The samples were frozen in liquid ethane (-183 A degrees C) and stored in liquid nitrogen. The specimens in the control group were not vitrified. Re-warming was performed at 40 A degrees C. The epithelial cell survival rate was assessed after 1, 3 and 7 days of storage following re-warming using calcein and ethidium homodimer-1 fluorescence. A wide range of values was observed among the different groups and among individual specimens within the groups. Resulting average survival rate was

Název v anglickém jazyce

Rapid cooling of the amniotic membrane as a model system for the vitrification of posterior corneal lamellae

Popis výsledku anglicky

To vitrify human amniotic membrane specimens so that the maximum of epithelial cells survives in order to develop a procedure for the eventual vitrification of posterior corneal lamellae without using cryoprotective agents. To assess different methods oftissue sample preparation preceding vitrification. In group 1, the amniotic membrane specimens were stretched on nitrocellulose support. In group 2, mechanical pressure was used to remove the excess culture medium between the support and the membrane. The samples were frozen in liquid ethane (-183 A degrees C) and stored in liquid nitrogen. The specimens in the control group were not vitrified. Re-warming was performed at 40 A degrees C. The epithelial cell survival rate was assessed after 1, 3 and 7 days of storage following re-warming using calcein and ethidium homodimer-1 fluorescence. A wide range of values was observed among the different groups and among individual specimens within the groups. Resulting average survival rate was

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GBP302%2F12%2FG157" target="_blank" >GBP302/12/G157: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cell and Tissue Banking

ISSN

1389-9333

e-ISSN

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Svazek periodika

15

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

165-173

Kód UT WoS článku

000332318700020

EID výsledku v databázi Scopus

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