Circulating tumor cells in pancreatic cancer patients: Enrichment and cultivation
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F14%3A10293161" target="_blank" >RIV/00216208:11110/14:10293161 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00216208:11120/14:43909243 RIV/00064173:_____/14:#0000394 RIV/00064203:_____/14:10293161
Výsledek na webu
<a href="http://dx.doi.org/10.3748/wjg.v20.i45.17163" target="_blank" >http://dx.doi.org/10.3748/wjg.v20.i45.17163</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3748/wjg.v20.i45.17163" target="_blank" >10.3748/wjg.v20.i45.17163</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Circulating tumor cells in pancreatic cancer patients: Enrichment and cultivation
Popis výsledku v původním jazyce
AIM: To investigate the feasibility of separation and cultivation of circulating tumor cells (CTCs) in pancreatic cancer (PaC) using a filtration device. METHODS: In total, 24 PaC patients who were candidates for surgical treatment were enrolled into thestudy. Peripheral blood samples were collected before an indicated surgery. For each patient, approximately 8 mL of venous blood was drawn from the antecubital veins. A new size-based separation MetaCell (R) technology was used for enrichment and cultivation of CTCs in vitro. (Separated CTCs were cultured on a membrane in FBS enriched RPMI media and observed by inverted microscope. The cultured cells were analyzed by means of histochemistry and immunohistochemistry using the specific antibodies to identify the cell origin. RESULTS: CTCs were detected in 16 patients (66.7%) of the 24 evaluable patients. The CTC positivity did not reflect the disease stage, tumor size, or lymph node involvement. The same percentage of CTC positivity was
Název v anglickém jazyce
Circulating tumor cells in pancreatic cancer patients: Enrichment and cultivation
Popis výsledku anglicky
AIM: To investigate the feasibility of separation and cultivation of circulating tumor cells (CTCs) in pancreatic cancer (PaC) using a filtration device. METHODS: In total, 24 PaC patients who were candidates for surgical treatment were enrolled into thestudy. Peripheral blood samples were collected before an indicated surgery. For each patient, approximately 8 mL of venous blood was drawn from the antecubital veins. A new size-based separation MetaCell (R) technology was used for enrichment and cultivation of CTCs in vitro. (Separated CTCs were cultured on a membrane in FBS enriched RPMI media and observed by inverted microscope. The cultured cells were analyzed by means of histochemistry and immunohistochemistry using the specific antibodies to identify the cell origin. RESULTS: CTCs were detected in 16 patients (66.7%) of the 24 evaluable patients. The CTC positivity did not reflect the disease stage, tumor size, or lymph node involvement. The same percentage of CTC positivity was
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
FD - Onkologie a hematologie
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/NT14439" target="_blank" >NT14439: Sledování hladiny cirkulujících nádorových buněk (CTC) u vybraných nádorů gastrointestinálního traktu</a><br>
Návaznosti
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Ostatní
Rok uplatnění
2014
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
World Journal of Gastroenterology
ISSN
1007-9327
e-ISSN
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Svazek periodika
20
Číslo periodika v rámci svazku
45
Stát vydavatele periodika
CN - Čínská lidová republika
Počet stran výsledku
8
Strana od-do
17163-17170
Kód UT WoS článku
000346050700038
EID výsledku v databázi Scopus
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