Proteomic analysis of the extracellular matrix in idiopathic pes equinovarus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F15%3A10288178" target="_blank" >RIV/00216208:11110/15:10288178 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985823:_____/15:00447679 RIV/00216208:11130/15:10288178

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s11010-014-2300-3" target="_blank" >http://dx.doi.org/10.1007/s11010-014-2300-3</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11010-014-2300-3" target="_blank" >10.1007/s11010-014-2300-3</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Proteomic analysis of the extracellular matrix in idiopathic pes equinovarus

Popis výsledku v původním jazyce

Idiopathic pes equinovarus is a congenital deformity of the foot and lower leg defined as a fixation of the foot in adduction, supination, and varus. Although the pathogenesis of clubfoot remains unclear, it has been suggested that fibroblasts and growthfactors are involved. To directly analyze the protein composition of the extracellular matrix in contracted tissue of patients with clubfoot. A total of 13 infants with idiopathic clubfoot treated with the Ponseti method were included in the present study. Tissue samples were obtained from patients undergoing surgery for relapsed clubfeet. Contracted tissues were obtained from the medial aspect of the talonavicular joint. Protein was extracted after digestion and delipidation using zip-tip C18. Individual collagenous fractions were detected using a chemiluminescent assay. Amino acid analysis of tissue samples revealed a predominance of collagens, namely collagen types I, III, and VI. The high content of glycine and h-proline suggests a

Název v anglickém jazyce

Proteomic analysis of the extracellular matrix in idiopathic pes equinovarus

Popis výsledku anglicky

Idiopathic pes equinovarus is a congenital deformity of the foot and lower leg defined as a fixation of the foot in adduction, supination, and varus. Although the pathogenesis of clubfoot remains unclear, it has been suggested that fibroblasts and growthfactors are involved. To directly analyze the protein composition of the extracellular matrix in contracted tissue of patients with clubfoot. A total of 13 infants with idiopathic clubfoot treated with the Ponseti method were included in the present study. Tissue samples were obtained from patients undergoing surgery for relapsed clubfeet. Contracted tissues were obtained from the medial aspect of the talonavicular joint. Protein was extracted after digestion and delipidation using zip-tip C18. Individual collagenous fractions were detected using a chemiluminescent assay. Amino acid analysis of tissue samples revealed a predominance of collagens, namely collagen types I, III, and VI. The high content of glycine and h-proline suggests a

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FI - Traumatologie a ortopedie

OECD FORD obor

—

Projekt

<a href="/cs/project/GA15-01948S" target="_blank" >GA15-01948S: Kapilární elektromigrační techniky využívající afinitní selektory a smart-polymery pro analýzu a studium vlastností a interakcí biomolekul.</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular and cellular biochemistry

ISSN

0300-8177

e-ISSN

—

Svazek periodika

401

Číslo periodika v rámci svazku

1-2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

133-139

Kód UT WoS článku

000352848900013

EID výsledku v databázi Scopus

2-s2.0-84925496363