Novel contribution to clubfoot pathogenesis: The possible role of extracellular matrix proteins

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F19%3A00504465" target="_blank" >RIV/67985823:_____/19:00504465 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11110/19:10391092 RIV/00216208:11130/19:10391092 RIV/00216208:11510/19:10391092 RIV/00216208:11310/19:10391092 a 2 dalších

Výsledek na webu

<a href="https://doi.org/10.1002/jor.24211" target="_blank" >https://doi.org/10.1002/jor.24211</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jor.24211" target="_blank" >10.1002/jor.24211</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Novel contribution to clubfoot pathogenesis: The possible role of extracellular matrix proteins

Popis výsledku v původním jazyce

Idiopathic pes equinovarus (clubfoot) is a congenital deformity of the feet and lower legs. Clubfoot belongs to a group of fibro‐proliferative disorders but its origin remains unknown. Our study aimed to achieve the first complex proteomic comparison of clubfoot contracted tissue of the foot (medial side, n = 16), with non‐contracted tissue (lateral side, n = 13). We used label‐free mass spectrometry quantification and immunohistochemistry. Seven proteins were observed to be significantly upregulated in the medial side (asporin, collagen type III, V, and VI, versican, tenascin‐C, and transforming growth factor beta induced protein) and four in the lateral side (collagen types XII and XIV, fibromodulin, and cartilage intermediate layer protein 2) of the clubfoot. Comparison of control samples from cadavers brought only two different protein concentrations (collagen types I and VI). We also revealed pathological calcification and intracellular positivity of transforming growth factor beta only in the contracted tissue of clubfoot. Most of the 11 differently expressed proteins are strongly related to the extracellular matrix architecture and we assume that they may play specific roles in the pathogenesis of this deformity. These proteins seem to be promising targets for future investigations and treatment of this disease.

Název v anglickém jazyce

Novel contribution to clubfoot pathogenesis: The possible role of extracellular matrix proteins

Popis výsledku anglicky

Idiopathic pes equinovarus (clubfoot) is a congenital deformity of the feet and lower legs. Clubfoot belongs to a group of fibro‐proliferative disorders but its origin remains unknown. Our study aimed to achieve the first complex proteomic comparison of clubfoot contracted tissue of the foot (medial side, n = 16), with non‐contracted tissue (lateral side, n = 13). We used label‐free mass spectrometry quantification and immunohistochemistry. Seven proteins were observed to be significantly upregulated in the medial side (asporin, collagen type III, V, and VI, versican, tenascin‐C, and transforming growth factor beta induced protein) and four in the lateral side (collagen types XII and XIV, fibromodulin, and cartilage intermediate layer protein 2) of the clubfoot. Comparison of control samples from cadavers brought only two different protein concentrations (collagen types I and VI). We also revealed pathological calcification and intracellular positivity of transforming growth factor beta only in the contracted tissue of clubfoot. Most of the 11 differently expressed proteins are strongly related to the extracellular matrix architecture and we assume that they may play specific roles in the pathogenesis of this deformity. These proteins seem to be promising targets for future investigations and treatment of this disease.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30211 - Orthopaedics

Projekt

<a href="/cs/project/NV17-31564A" target="_blank" >NV17-31564A: Aplikace látek pro zefektivnění neinvazivní léčby fibrózního stavu u onemocnění pes equinovarus</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Orthopaedic Research

ISSN

0736-0266

e-ISSN

—

Svazek periodika

37

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

769-778

Kód UT WoS článku

000464419100026

EID výsledku v databázi Scopus

2-s2.0-85061451671