Protective effect of heme oxygenase induction in ethinylestradiol-induced cholestasis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F15%3A10295100" target="_blank" >RIV/00216208:11110/15:10295100 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11150/15:10295100 RIV/00216208:11160/15:10295100 RIV/00064165:_____/15:10295100

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1111/jcmm.12401/full" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/jcmm.12401/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/jcmm.12401" target="_blank" >10.1111/jcmm.12401</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Protective effect of heme oxygenase induction in ethinylestradiol-induced cholestasis

Popis výsledku v původním jazyce

Estrogen-induced cholestasis is characterized by impaired hepatic uptake and biliary bile acids secretion because of changes in hepatocyte transporter expression. The induction of heme oxygenase-1 (HMOX1), the inducible isozyme in heme catabolism, is mediated via the Bach1/Nrf2 pathway, and protects livers from toxic, oxidative and inflammatory insults. However, its role in cholestasis remains unknown. Here, we investigated the effects of HMOX1 induction by heme on ethinylestradiol(EE)-induced cholestasis and possible underlying mechanisms. Wistar rats were given EE (5 mg/kg s.c.) for 5 days. HMOX1 was induced by heme (15 mu mol/kg i.p.) 24 hrs prior to EE. Serum cholestatic markers, hepatocyte and renal membrane transporter expression, and biliary andurinary bile acids excretion were quantified. EE significantly increased cholestatic markers (P<=0.01), decreased biliary bile acid excretion (39%, P=0.01), down-regulated hepatocyte transporters (Ntcp/Oatp1b2/Oatp1a4/Mrp2, P<=0.05), and

Název v anglickém jazyce

Protective effect of heme oxygenase induction in ethinylestradiol-induced cholestasis

Popis výsledku anglicky

Estrogen-induced cholestasis is characterized by impaired hepatic uptake and biliary bile acids secretion because of changes in hepatocyte transporter expression. The induction of heme oxygenase-1 (HMOX1), the inducible isozyme in heme catabolism, is mediated via the Bach1/Nrf2 pathway, and protects livers from toxic, oxidative and inflammatory insults. However, its role in cholestasis remains unknown. Here, we investigated the effects of HMOX1 induction by heme on ethinylestradiol(EE)-induced cholestasis and possible underlying mechanisms. Wistar rats were given EE (5 mg/kg s.c.) for 5 days. HMOX1 was induced by heme (15 mu mol/kg i.p.) 24 hrs prior to EE. Serum cholestatic markers, hepatocyte and renal membrane transporter expression, and biliary andurinary bile acids excretion were quantified. EE significantly increased cholestatic markers (P<=0.01), decreased biliary bile acid excretion (39%, P=0.01), down-regulated hepatocyte transporters (Ntcp/Oatp1b2/Oatp1a4/Mrp2, P<=0.05), and

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FE - Ostatní obory vnitřního lékařství

OECD FORD obor

—

Projekt

<a href="/cs/project/NT11327" target="_blank" >NT11327: Role hemoxygenázy v patogenezi cholestázy</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Cellular and Molecular Medicine

ISSN

1582-4934

e-ISSN

—

Svazek periodika

19

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

10

Strana od-do

924-933

Kód UT WoS článku

000353991100003

EID výsledku v databázi Scopus

2-s2.0-84928430781