Localized movement and morphology of UBF1-positive nucleolar regions are changed by gamma-irradiation in G2 phase of the cell cycle

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F15%3A10297886" target="_blank" >RIV/00216208:11110/15:10297886 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14330/15:00080738

Výsledek na webu

<a href="http://dx.doi.org/10.1080/19491034.2015.1075111" target="_blank" >http://dx.doi.org/10.1080/19491034.2015.1075111</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/19491034.2015.1075111" target="_blank" >10.1080/19491034.2015.1075111</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Localized movement and morphology of UBF1-positive nucleolar regions are changed by gamma-irradiation in G2 phase of the cell cycle

Popis výsledku v původním jazyce

The nucleolus is a well-organized site of ribosomal gene transcription. Moreover, many DNA repair pathway proteins, including ATM, ATR kinases, MRE11, PARP1 and Ku70/80, localize to the nucleolus (Moore et al., 2011). We analyzed the consequences of DNAdamage in nucleoli following ultraviolet A (UVA), C (UVC), or gamma-irradiation in order to test whether and how radiation-mediated genome injury affects local motion and morphology of nucleoli. Because exposure to radiation sources can induce changes inthe pattern of UBF1-positive nucleolar regions, we visualized nucleoli in living cells by GFP-UBF1 expression for subsequent morphological analyses and local motion studies. UVA radiation, but not 5 Gy of gamma-rays, induced apoptosis as analyzed by anadvanced computational method. In non-apoptotic cells, we observed that gamma-radiation caused nucleolar re-positioning over time and changed several morphological parameters, including the size of the nucleolus and the area of individual

Název v anglickém jazyce

Localized movement and morphology of UBF1-positive nucleolar regions are changed by gamma-irradiation in G2 phase of the cell cycle

Popis výsledku anglicky

The nucleolus is a well-organized site of ribosomal gene transcription. Moreover, many DNA repair pathway proteins, including ATM, ATR kinases, MRE11, PARP1 and Ku70/80, localize to the nucleolus (Moore et al., 2011). We analyzed the consequences of DNAdamage in nucleoli following ultraviolet A (UVA), C (UVC), or gamma-irradiation in order to test whether and how radiation-mediated genome injury affects local motion and morphology of nucleoli. Because exposure to radiation sources can induce changes inthe pattern of UBF1-positive nucleolar regions, we visualized nucleoli in living cells by GFP-UBF1 expression for subsequent morphological analyses and local motion studies. UVA radiation, but not 5 Gy of gamma-rays, induced apoptosis as analyzed by anadvanced computational method. In non-apoptotic cells, we observed that gamma-radiation caused nucleolar re-positioning over time and changed several morphological parameters, including the size of the nucleolus and the area of individual

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

—

Projekt

<a href="/cs/project/GBP302%2F12%2FG157" target="_blank" >GBP302/12/G157: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii</a><br>

Návaznosti

O - Projekt operacniho programu

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nucleus

ISSN

1949-1034

e-ISSN

—

Svazek periodika

6

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

13

Strana od-do

301-313

Kód UT WoS článku

000360666000008

EID výsledku v databázi Scopus

2-s2.0-84954480958