Localized movement and morphology of UBF1-positive nucleolar regions are changed by -irradiation in G2 phase of the cell cycle

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F15%3A00456708" target="_blank" >RIV/68081707:_____/15:00456708 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1080/19491034.2015.1075111" target="_blank" >http://dx.doi.org/10.1080/19491034.2015.1075111</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/19491034.2015.1075111" target="_blank" >10.1080/19491034.2015.1075111</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Localized movement and morphology of UBF1-positive nucleolar regions are changed by -irradiation in G2 phase of the cell cycle

Popis výsledku v původním jazyce

The nucleolus is a well-organized site of ribosomal gene transcription. Moreover, many DNA repair pathway proteins, including ATM, ATR kinases, MRE11, PARP1 and Ku70/80, localize to the nucleolus (Moore etal., 2011). We analyzed the consequences of DNA damage in nucleoli following ultraviolet A (UVA), C (UVC), or -irradiation in order to test whether and how radiation-mediated genome injury affects local motion and morphology of nucleoli. Because exposure to radiation sources can induce changes in the pattern of UBF1-positive nucleolar regions, we visualized nucleoli in living cells by GFP-UBF1 expression for subsequent morphological analyses and local motion studies. UVA radiation, but not 5Gy of -rays, induced apoptosis as analyzed by an advanced computational method. In non-apoptotic cells, we observed that -radiation caused nucleolar re-positioning over time and changed several morphological parameters, including the size of the nucleolus and the area of individual UBF1-positive fo

Název v anglickém jazyce

Localized movement and morphology of UBF1-positive nucleolar regions are changed by -irradiation in G2 phase of the cell cycle

Popis výsledku anglicky

The nucleolus is a well-organized site of ribosomal gene transcription. Moreover, many DNA repair pathway proteins, including ATM, ATR kinases, MRE11, PARP1 and Ku70/80, localize to the nucleolus (Moore etal., 2011). We analyzed the consequences of DNA damage in nucleoli following ultraviolet A (UVA), C (UVC), or -irradiation in order to test whether and how radiation-mediated genome injury affects local motion and morphology of nucleoli. Because exposure to radiation sources can induce changes in the pattern of UBF1-positive nucleolar regions, we visualized nucleoli in living cells by GFP-UBF1 expression for subsequent morphological analyses and local motion studies. UVA radiation, but not 5Gy of -rays, induced apoptosis as analyzed by an advanced computational method. In non-apoptotic cells, we observed that -radiation caused nucleolar re-positioning over time and changed several morphological parameters, including the size of the nucleolus and the area of individual UBF1-positive fo

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nucleus

ISSN

1949-1034

e-ISSN

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Svazek periodika

6

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

13

Strana od-do

301-313

Kód UT WoS článku

000360666000008

EID výsledku v databázi Scopus

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