Isolation, primary culture, morphological and molecular characterization of circulating tumor cells in gynecological cancers

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F15%3A10301827" target="_blank" >RIV/00216208:11110/15:10301827 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00064173:_____/15:#0000453 RIV/00064203:_____/15:10301827

Výsledek na webu

<a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4548313/pdf/ajtr0007-1203.pdf" target="_blank" >http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4548313/pdf/ajtr0007-1203.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Isolation, primary culture, morphological and molecular characterization of circulating tumor cells in gynecological cancers

Popis výsledku v původním jazyce

The focus of the study was to implement a new workflow for circulating tumor cells (CTCs) characterization that would allow the analysis of CTCs on a cytomorphological and molecular level in patients with diagnosed gynecological cancer. Our findings maybe useful in future cancer patient management. The study introduces a size-based enrichment (MetaCell (R)) method for the separation of viable CTCs, followed by CTCs culturing in vitro and gene expression characterization. It is based on the observationof CTCs and DTCs (Disseminated Tumor Cells) in several case studies of ovarian, endometrial and cervical cancer by means of cytomorphology and gene expression profiling. The viability of the enriched CTCs was estimated using vital and lethal fluorescencenuclear staining. This type of staining may be predictive for the success rate of subsequent CTC growth in vitro. To identify CTCs in the enriched CTC fraction, cytomorphological evaluations based on vital fluorescence staining were foll

Název v anglickém jazyce

Isolation, primary culture, morphological and molecular characterization of circulating tumor cells in gynecological cancers

Popis výsledku anglicky

The focus of the study was to implement a new workflow for circulating tumor cells (CTCs) characterization that would allow the analysis of CTCs on a cytomorphological and molecular level in patients with diagnosed gynecological cancer. Our findings maybe useful in future cancer patient management. The study introduces a size-based enrichment (MetaCell (R)) method for the separation of viable CTCs, followed by CTCs culturing in vitro and gene expression characterization. It is based on the observationof CTCs and DTCs (Disseminated Tumor Cells) in several case studies of ovarian, endometrial and cervical cancer by means of cytomorphology and gene expression profiling. The viability of the enriched CTCs was estimated using vital and lethal fluorescencenuclear staining. This type of staining may be predictive for the success rate of subsequent CTC growth in vitro. To identify CTCs in the enriched CTC fraction, cytomorphological evaluations based on vital fluorescence staining were foll

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

<a href="/cs/project/NT14441" target="_blank" >NT14441: Cirkulující a diseminované nádorové buňky v diagnostice a léčbě pokročilých stadií karcinomu ovaria</a><br>

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

American Journal of Translational Research

ISSN

1943-8141

e-ISSN

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Svazek periodika

7

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

1203-1213

Kód UT WoS článku

000361558000002

EID výsledku v databázi Scopus

2-s2.0-84939535871