Expression of the cellular prion protein affects posttransfusion recovery and survival of red blood cells in mice

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F15%3A10313286" target="_blank" >RIV/00216208:11110/15:10313286 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1111/trf.13190" target="_blank" >http://dx.doi.org/10.1111/trf.13190</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/trf.13190" target="_blank" >10.1111/trf.13190</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Expression of the cellular prion protein affects posttransfusion recovery and survival of red blood cells in mice

Popis výsledku v původním jazyce

BACKGROUNDCellular prion protein (PrPC) is expressed on various cell types including red blood cells (RBCs). The PrPC plays a key role in the pathogenesis of prion diseases, but its physiologic function remains unclear. PrPC is expressed on CD34+ hematopoietic stem cells and its expression is regulated during blood cell differentiation including the erythroid line. STUDY DESIGN AND METHODSWe investigated the role of PrPC in RBC survival in circulation by transfusing a mix of biotin-labeled RBCs from wild-type (WT) and PrP knockout (KO) mice to groups of recipient mice (WT and KO). The proportion of biotinylated RBCs in peripheral blood was estimated by flow cytometry. RESULTSKO RBCs displayed a markedly higher first-day posttransfusion recovery but hada decreased survival in circulation when compared to WT RBCs. Similar results were obtained in all groups of transfused mice, irrespective of RBCs biotinylation level. In addition, we confirmed this finding in an analogous study using Tg

Název v anglickém jazyce

Expression of the cellular prion protein affects posttransfusion recovery and survival of red blood cells in mice

Popis výsledku anglicky

BACKGROUNDCellular prion protein (PrPC) is expressed on various cell types including red blood cells (RBCs). The PrPC plays a key role in the pathogenesis of prion diseases, but its physiologic function remains unclear. PrPC is expressed on CD34+ hematopoietic stem cells and its expression is regulated during blood cell differentiation including the erythroid line. STUDY DESIGN AND METHODSWe investigated the role of PrPC in RBC survival in circulation by transfusing a mix of biotin-labeled RBCs from wild-type (WT) and PrP knockout (KO) mice to groups of recipient mice (WT and KO). The proportion of biotinylated RBCs in peripheral blood was estimated by flow cytometry. RESULTSKO RBCs displayed a markedly higher first-day posttransfusion recovery but hada decreased survival in circulation when compared to WT RBCs. Similar results were obtained in all groups of transfused mice, irrespective of RBCs biotinylation level. In addition, we confirmed this finding in an analogous study using Tg

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

<a href="/cs/project/GAP303%2F12%2F1791" target="_blank" >GAP303/12/1791: Úloha proteázami aktivovaných receptorů v patogenezi prionových chorob</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Transfusion

ISSN

0041-1132

e-ISSN

—

Svazek periodika

55

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

2590-2596

Kód UT WoS článku

000364876100010

EID výsledku v databázi Scopus

2-s2.0-84947087470