Study of silica-based electrospun nanofibers as a scaffold for human bone marrow mesenchymal stem cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F15%3A10323239" target="_blank" >RIV/00216208:11110/15:10323239 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.nanocon.eu/files/proceedings/23/papers/4483.pdf" target="_blank" >http://www.nanocon.eu/files/proceedings/23/papers/4483.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Study of silica-based electrospun nanofibers as a scaffold for human bone marrow mesenchymal stem cells

Popis výsledku v původním jazyce

Electrospun nanofibers with their nanoscale structure and ability to mimic native extracellular matrix (ECM) harbor a great potential for biotechnology, biomedicine, and tissue engineering. Inorganic nanofibers, such as silica-based nanofibers, have attracted attention for their physical and chemical stability, surface functionalities and hydrophilic nature. In this study, we tested biodegradable SiO2 nanofibers prepared by sol-gel method and needleless electrospinning as a scaffold for human bone marrow mesenchymal stem cells (hMSC), cells which are promising for regenerative medicine. The nanofibers were tested for cytotoxicity and capability to support cell adhesion. For cytotoxicity testing, the nanofibers were incubated for 24 or 72 hours in phosphate-buffered saline (PBS) or cell culture media supplemented with serum. The eluate was then applied to hMSC for either 24 hours or 4 days and cytotoxicity was evaluated using MTT assay. While eluates from PBS-treated nanofibers had no e

Název v anglickém jazyce

Study of silica-based electrospun nanofibers as a scaffold for human bone marrow mesenchymal stem cells

Popis výsledku anglicky

Electrospun nanofibers with their nanoscale structure and ability to mimic native extracellular matrix (ECM) harbor a great potential for biotechnology, biomedicine, and tissue engineering. Inorganic nanofibers, such as silica-based nanofibers, have attracted attention for their physical and chemical stability, surface functionalities and hydrophilic nature. In this study, we tested biodegradable SiO2 nanofibers prepared by sol-gel method and needleless electrospinning as a scaffold for human bone marrow mesenchymal stem cells (hMSC), cells which are promising for regenerative medicine. The nanofibers were tested for cytotoxicity and capability to support cell adhesion. For cytotoxicity testing, the nanofibers were incubated for 24 or 72 hours in phosphate-buffered saline (PBS) or cell culture media supplemented with serum. The eluate was then applied to hMSC for either 24 hours or 4 days and cytotoxicity was evaluated using MTT assay. While eluates from PBS-treated nanofibers had no e

Druh

D - Stať ve sborníku

CEP obor

EA - Morfologické obory a cytologie

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

NANOCON 2015 - 7th International Conference on Nanomaterials - Research and Application, Conference Proceedings

ISBN

978-80-87294-63-5

ISSN

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e-ISSN

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Počet stran výsledku

5

Strana od-do

419-423

Název nakladatele

TANGER Ltd.

Místo vydání

Ostrava

Místo konání akce

Brno

Datum konání akce

14. 10. 2015

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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