Hyaluronic acid in complexes with surfactants: The efficient tool for reduction of the cytotoxic effect of surfactants on human cell types

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F17%3A10360172" target="_blank" >RIV/00216208:11110/17:10360172 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11140/17:10360172 RIV/00216305:26310/17:PU124165

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S014181301731317X" target="_blank" >http://www.sciencedirect.com/science/article/pii/S014181301731317X</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ijbiomac.2017.05.173" target="_blank" >10.1016/j.ijbiomac.2017.05.173</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Hyaluronic acid in complexes with surfactants: The efficient tool for reduction of the cytotoxic effect of surfactants on human cell types

Popis výsledku v původním jazyce

tThe cationic surfactants carbethoxypendecinium bromide (Septonex) and cetyltrimethylammonium bro-mide (CTAB) are known to be harmful for certain cell types (bacteria, fungi, mammal cells, etc.). Colloidalcomplexes of these surfactants with negatively-charged hyaluronic acid (HyA) were prepared for poten-tial drug and/or universal delivery applications. The complexes were tested for their cytotoxic effect ondifferent human cell types - osteoblasts, keratinocytes and fibroblasts. Both the CTAB-HyA and Septonex-HyA complexes were found to reduce the cytotoxicity induced by surfactants alone concerning all thetested concentrations. Moreover, we suggested the limits of HyA protection provided by the surfactant-HyA complexes, e.g. the importance of the amount of HyA applied. We also determined the specificsensitivity of different cell types to surfactant treatment. Keratinocytes were more sensitive to CTAB,while osteoblasts and fibroblasts were more sensitive to Septonex. Moreover, it was indirectly shownthat CTAB combines lethal toxicity with cell metabolism induction, while Septonex predominantly causeslethal toxicity concerning fibroblasts. This comprehensive study of the effect of surfactant-HyA com-plexes on various human cell types revealed that HyA represents a useful CTAB or Septonex cytotoxiceffect modulator at diverse levels. Potential applications for these complexes include drug and/or nucleicacid delivery systems, diagnostic dye carriers and cosmetics production.

Název v anglickém jazyce

Hyaluronic acid in complexes with surfactants: The efficient tool for reduction of the cytotoxic effect of surfactants on human cell types

Popis výsledku anglicky

tThe cationic surfactants carbethoxypendecinium bromide (Septonex) and cetyltrimethylammonium bro-mide (CTAB) are known to be harmful for certain cell types (bacteria, fungi, mammal cells, etc.). Colloidalcomplexes of these surfactants with negatively-charged hyaluronic acid (HyA) were prepared for poten-tial drug and/or universal delivery applications. The complexes were tested for their cytotoxic effect ondifferent human cell types - osteoblasts, keratinocytes and fibroblasts. Both the CTAB-HyA and Septonex-HyA complexes were found to reduce the cytotoxicity induced by surfactants alone concerning all thetested concentrations. Moreover, we suggested the limits of HyA protection provided by the surfactant-HyA complexes, e.g. the importance of the amount of HyA applied. We also determined the specificsensitivity of different cell types to surfactant treatment. Keratinocytes were more sensitive to CTAB,while osteoblasts and fibroblasts were more sensitive to Septonex. Moreover, it was indirectly shownthat CTAB combines lethal toxicity with cell metabolism induction, while Septonex predominantly causeslethal toxicity concerning fibroblasts. This comprehensive study of the effect of surfactant-HyA com-plexes on various human cell types revealed that HyA represents a useful CTAB or Septonex cytotoxiceffect modulator at diverse levels. Potential applications for these complexes include drug and/or nucleicacid delivery systems, diagnostic dye carriers and cosmetics production.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10404 - Polymer science

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Biological Macromolecules

ISSN

0141-8130

e-ISSN

—

Svazek periodika

103

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

1276-1284

Kód UT WoS článku

000408286400143

EID výsledku v databázi Scopus

2-s2.0-85020248482