Comparison of DNA isolation using salting-out procedure and automated isolation (MagNA system)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F17%3A10362277" target="_blank" >RIV/00216208:11110/17:10362277 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00064165:_____/17:10362277

Výsledek na webu

<a href="http://dx.doi.org/10.1080/10826068.2017.1303613" target="_blank" >http://dx.doi.org/10.1080/10826068.2017.1303613</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/10826068.2017.1303613" target="_blank" >10.1080/10826068.2017.1303613</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of DNA isolation using salting-out procedure and automated isolation (MagNA system)

Popis výsledku v původním jazyce

Isolation of genomic DNA is a key step in genetic analysis. The aim of the study was to evaluate the suitability of isolation of DNA from peripheral blood with manual salting-out procedure and automated MagNA system under specific conditions. The impact of storage conditions, type of material (whole blood or blood cells), and method used for DNA extraction were evaluated in terms of DNA yield, its purity, and integrity. Fresh material, and material stored at 2-8 degrees C for 1-4 weeks and frozen at -80 degrees C were tested. For fresh samples, salting-out method gives higher yield than MagNA, irrespectively, on material used. Neither the yield of salting-out method nor its purity decreases during the storage of the samples in the fridge (2-8 degrees C) during 4 weeks. Concerning MagNA, storage of blood cells in the fridge decreases the yield of DNA as well as its purity. For frozen samples, for whole blood, MagNA gives better results while for blood cells, salting-out method seems to be better. For fresh samples, salting-out method is the preferred one, and both whole blood and blood cells can be used. For frozen samples, the preferred method depends on the material.

Název v anglickém jazyce

Comparison of DNA isolation using salting-out procedure and automated isolation (MagNA system)

Popis výsledku anglicky

Isolation of genomic DNA is a key step in genetic analysis. The aim of the study was to evaluate the suitability of isolation of DNA from peripheral blood with manual salting-out procedure and automated MagNA system under specific conditions. The impact of storage conditions, type of material (whole blood or blood cells), and method used for DNA extraction were evaluated in terms of DNA yield, its purity, and integrity. Fresh material, and material stored at 2-8 degrees C for 1-4 weeks and frozen at -80 degrees C were tested. For fresh samples, salting-out method gives higher yield than MagNA, irrespectively, on material used. Neither the yield of salting-out method nor its purity decreases during the storage of the samples in the fridge (2-8 degrees C) during 4 weeks. Concerning MagNA, storage of blood cells in the fridge decreases the yield of DNA as well as its purity. For frozen samples, for whole blood, MagNA gives better results while for blood cells, salting-out method seems to be better. For fresh samples, salting-out method is the preferred one, and both whole blood and blood cells can be used. For frozen samples, the preferred method depends on the material.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/LM2015089" target="_blank" >LM2015089: Banka klinických vzorků</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Preparative Biochemistry and Biotechnology

ISSN

1082-6068

e-ISSN

—

Svazek periodika

47

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

703-708

Kód UT WoS článku

000407899900009

EID výsledku v databázi Scopus

2-s2.0-85017099093