Human polyomavirus 6 and 7 are associated with pruritic and dyskeratotic dermatoses

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F17%3A10363704" target="_blank" >RIV/00216208:11110/17:10363704 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00064165:_____/17:10363704

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.jaad.2016.11.035" target="_blank" >http://dx.doi.org/10.1016/j.jaad.2016.11.035</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jaad.2016.11.035" target="_blank" >10.1016/j.jaad.2016.11.035</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Human polyomavirus 6 and 7 are associated with pruritic and dyskeratotic dermatoses

Popis výsledku v původním jazyce

Background: Human polyomavirus (HPyV) 6 and HPyV7 are shed chronically from human skin. HPyV7, but not HPyV6, has been linked to a pruritic skin eruption of immunosuppression. Objective: We determined whether biopsy specimens showing a characteristic pattern of dyskeratosis and parakeratosis might be associated with polyomavirus infection. Methods: We screened biopsy specimens showing &quot;peacock plumage&apos;&apos; histology by polymerase chain reaction for HPyVs. Cases positive for HPyV6 or HPyV7 were then analyzed by immunohistochemistry, electron microscopy, immunofluorescence, quantitative polymerase chain reaction, and complete sequencing, including unbiased, next-generation sequencing. Results: We identified 3 additional cases of HPyV6 or HPyV7 skin infections. Expression of T antigen and viral capsid was abundant in lesional skin. Dual immunofluorescence staining experiments confirmed that HPyV7 primarily infects keratinocytes. High viral loads in lesional skin compared with normal-appearing skin and the identification of intact virions by both electron microscopy and next-generation sequencing support a role for active viral infections in these skin diseases. Limitation: This was a small case series of archived materials. Conclusion: We have found that HPyV6 and HPyV7 are associated with rare, pruritic skin eruptions with a distinctive histologic pattern and describe this entity as &quot;HPyV6-and HPyV7-associated pruritic and dyskeratotic dermatoses.&apos;&apos;

Název v anglickém jazyce

Human polyomavirus 6 and 7 are associated with pruritic and dyskeratotic dermatoses

Popis výsledku anglicky

Background: Human polyomavirus (HPyV) 6 and HPyV7 are shed chronically from human skin. HPyV7, but not HPyV6, has been linked to a pruritic skin eruption of immunosuppression. Objective: We determined whether biopsy specimens showing a characteristic pattern of dyskeratosis and parakeratosis might be associated with polyomavirus infection. Methods: We screened biopsy specimens showing &quot;peacock plumage&apos;&apos; histology by polymerase chain reaction for HPyVs. Cases positive for HPyV6 or HPyV7 were then analyzed by immunohistochemistry, electron microscopy, immunofluorescence, quantitative polymerase chain reaction, and complete sequencing, including unbiased, next-generation sequencing. Results: We identified 3 additional cases of HPyV6 or HPyV7 skin infections. Expression of T antigen and viral capsid was abundant in lesional skin. Dual immunofluorescence staining experiments confirmed that HPyV7 primarily infects keratinocytes. High viral loads in lesional skin compared with normal-appearing skin and the identification of intact virions by both electron microscopy and next-generation sequencing support a role for active viral infections in these skin diseases. Limitation: This was a small case series of archived materials. Conclusion: We have found that HPyV6 and HPyV7 are associated with rare, pruritic skin eruptions with a distinctive histologic pattern and describe this entity as &quot;HPyV6-and HPyV7-associated pruritic and dyskeratotic dermatoses.&apos;&apos;

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30216 - Dermatology and venereal diseases

Projekt

—

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of the American Academy of Dermatology

ISSN

0190-9622

e-ISSN

—

Svazek periodika

76

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

932-940

Kód UT WoS článku

000399217200037

EID výsledku v databázi Scopus

2-s2.0-85009380248