Structural changes arising from different thawing protocols on cryopreserved human allograft's aortic valve leaflets

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F18%3A10378537" target="_blank" >RIV/00216208:11110/18:10378537 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11130/18:10378537 RIV/00216208:11150/18:10378537 RIV/00179906:_____/18:10378537 RIV/00064203:_____/18:10378537 a 2 dalších

Výsledek na webu

<a href="https://doi.org/10.17219/acem/73713" target="_blank" >https://doi.org/10.17219/acem/73713</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17219/acem/73713" target="_blank" >10.17219/acem/73713</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Structural changes arising from different thawing protocols on cryopreserved human allograft's aortic valve leaflets

Popis výsledku v původním jazyce

Background. The aim of our experimental work was to assess the impact and morphological changes that arise during different thawing protocols on human aortic valve (AV) leaflets resected from cryopreserved aortic root allog rafts (CARAs). Objectives. Two thawing protocols were tested: 1. CARAs were thawed at a room temperature (23 degrees C); 2. CARAs were placed directly into a water bath at a temperature of 37 degrees C. After all the samples were thawed, non-coronary AV leaflets were sampled from each specimen and fixed in a 4% formaldehyde solution before they were sent for morphological analysis. Material and methods. All the samples were washed in distilled water for 5 min and dehydrated in a graded ethanol series (70%, 85%, 95%, and 100%) for 5 min at each level. The tissue samples were then immersed in 100% hexamethyldisilazane (HMDS) for 10 min, and then air-dried in an exhaust hood at room temperature. Processed samples were mounted on stainless steel stubs and coated with gold. Histological analysis was performed with the use of an electron microscope on a scanning mode operating at 25 kV - BS 301. Results. Thawing protocol 1 (room temperature at 23 degrees C): 6 (100%) samples showed loss of the endothelial covering of the basal membrane with no damage to the basal lamina. Thawing protocol 2 (water bath at 37 degrees C): 5 (83%) samples showed loss of the endothelial covering of the basal membrane with no damage to the basal lamina. One (17%) sample showed loss of the endothelial covering the basal membrane with significant damage to the basal membrane. Conclusions. Based on our experimental work, we can clearly conclude that cryopreserved AV leaflet allografts show identical structural changes at different rates of thawing.

Název v anglickém jazyce

Structural changes arising from different thawing protocols on cryopreserved human allograft's aortic valve leaflets

Popis výsledku anglicky

Background. The aim of our experimental work was to assess the impact and morphological changes that arise during different thawing protocols on human aortic valve (AV) leaflets resected from cryopreserved aortic root allog rafts (CARAs). Objectives. Two thawing protocols were tested: 1. CARAs were thawed at a room temperature (23 degrees C); 2. CARAs were placed directly into a water bath at a temperature of 37 degrees C. After all the samples were thawed, non-coronary AV leaflets were sampled from each specimen and fixed in a 4% formaldehyde solution before they were sent for morphological analysis. Material and methods. All the samples were washed in distilled water for 5 min and dehydrated in a graded ethanol series (70%, 85%, 95%, and 100%) for 5 min at each level. The tissue samples were then immersed in 100% hexamethyldisilazane (HMDS) for 10 min, and then air-dried in an exhaust hood at room temperature. Processed samples were mounted on stainless steel stubs and coated with gold. Histological analysis was performed with the use of an electron microscope on a scanning mode operating at 25 kV - BS 301. Results. Thawing protocol 1 (room temperature at 23 degrees C): 6 (100%) samples showed loss of the endothelial covering of the basal membrane with no damage to the basal lamina. Thawing protocol 2 (water bath at 37 degrees C): 5 (83%) samples showed loss of the endothelial covering of the basal membrane with no damage to the basal lamina. One (17%) sample showed loss of the endothelial covering the basal membrane with significant damage to the basal membrane. Conclusions. Based on our experimental work, we can clearly conclude that cryopreserved AV leaflet allografts show identical structural changes at different rates of thawing.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30201 - Cardiac and Cardiovascular systems

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Advances in Clinical and Experimental Medicine

ISSN

1899-5276

e-ISSN

—

Svazek periodika

27

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

PL - Polská republika

Počet stran výsledku

4

Strana od-do

1033-1036

Kód UT WoS článku

000443031700002

EID výsledku v databázi Scopus

2-s2.0-85052986911