Optimization of the photodynamic inactivation of prions by a phthalocyanine photosensitizer: The crucial involvement of singlet oxygen

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F19%3A10396769" target="_blank" >RIV/00216208:11110/19:10396769 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/19:10396769 RIV/49777513:23220/19:43955399

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=Bo5hM53Rwc" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=Bo5hM53Rwc</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jbio.201800430" target="_blank" >10.1002/jbio.201800430</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Optimization of the photodynamic inactivation of prions by a phthalocyanine photosensitizer: The crucial involvement of singlet oxygen

Popis výsledku v původním jazyce

Prion disorders are fatal neurodegenerative diseases caused by the autocatalytic conversion of a natively occurring prion protein (PrP (c)) into its misfolded infectious form (PrPTSE). The proven resistance of PrPTSE to common disinfection procedures increases the risk of prion transmission in medical settings. Herein, we present the effective photodynamic inactivation (PDI) of prions by disulfonated hydroxyaluminum phthalocyanine (AlPcOH(SO3)(2)) utilizing two custom-built red light sources. The treatment eliminates PrPTSE signal in infectious mouse brain homogenate with efficiency that depends on light intensity but has a low effect on the overall protein content. Importantly, singlet oxygen (O-2((1)Delta(g))) is the only species significantly photogenerated by AlPcOH(SO3)(2), and it is responsible for the PDI of prions. More intensive light conditions show not only higher O-2((1)Delta(g)) production but also decreases in AlPcOH(SO3)(2) photostability. Our findings suggest that PDI by AlPcOH(SO3)(2)-generated O-2((1)Delta(g)) represents a promising approach for prion inactivation that may be useful in future decontamination strategies for delicate medical tools.

Název v anglickém jazyce

Optimization of the photodynamic inactivation of prions by a phthalocyanine photosensitizer: The crucial involvement of singlet oxygen

Popis výsledku anglicky

Prion disorders are fatal neurodegenerative diseases caused by the autocatalytic conversion of a natively occurring prion protein (PrP (c)) into its misfolded infectious form (PrPTSE). The proven resistance of PrPTSE to common disinfection procedures increases the risk of prion transmission in medical settings. Herein, we present the effective photodynamic inactivation (PDI) of prions by disulfonated hydroxyaluminum phthalocyanine (AlPcOH(SO3)(2)) utilizing two custom-built red light sources. The treatment eliminates PrPTSE signal in infectious mouse brain homogenate with efficiency that depends on light intensity but has a low effect on the overall protein content. Importantly, singlet oxygen (O-2((1)Delta(g))) is the only species significantly photogenerated by AlPcOH(SO3)(2), and it is responsible for the PDI of prions. More intensive light conditions show not only higher O-2((1)Delta(g)) production but also decreases in AlPcOH(SO3)(2) photostability. Our findings suggest that PDI by AlPcOH(SO3)(2)-generated O-2((1)Delta(g)) represents a promising approach for prion inactivation that may be useful in future decontamination strategies for delicate medical tools.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30102 - Immunology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Biophotonics

ISSN

1864-063X

e-ISSN

—

Svazek periodika

12

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

13

Strana od-do

e201800340

Kód UT WoS článku

000480614900020

EID výsledku v databázi Scopus

2-s2.0-85065342147