A novel accurate LC-MS/MS method for quantitative determination of Z-lumirubin

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F20%3A10414639" target="_blank" >RIV/00216208:11110/20:10414639 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/20:00117827 RIV/00064165:_____/20:10414639

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=qiWh~yLIYZ" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=qiWh~yLIYZ</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-020-61280-z" target="_blank" >10.1038/s41598-020-61280-z</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A novel accurate LC-MS/MS method for quantitative determination of Z-lumirubin

Popis výsledku v původním jazyce

Although phototherapy (PT) is a standard treatment for neonatal jaundice, no validated clinical methods for determination of bilirubin phototherapy products are available. Thus, the aim of our study was to establish a such method for clinical use. To achieve this aim, a LC-MS/MS assay for simultaneous determination of Z-lumirubin (LR) and unconjugated bilirubin (UCB) was conducted. LR was purified after irradiation of UCB at 460 nm. The assay was tested on human sera from PT-treated neonates. Samples were separated on a HPLC system with a triple quadrupole mass spectrometer detector. The instrument response was linear up to 5.8 and 23.4 mg/dL for LR and UCB, respectively, with submicromolar limits of detection and validity parameters relevant for use in clinical medicine. Exposure of newborns to PT raised serum LR concentrations three-fold (p &lt; 0.01), but the absolute concentrations were low (0.37 +/- 0.16 mg/dL), despite a dramatic decrease of serum UCB concentrations (13.6 +/- 2.2 vs. 10.3 +/- 3.3 mg/dL, p &lt; 0.01). A LC-MS/MS method for the simultaneous determination of LR and UCB in human serum was established and validated for clinical use. This method should help to monitor neonates on PT, as well as to improve our understanding of both the kinetics and biology of bilirubin phototherapy products.

Název v anglickém jazyce

A novel accurate LC-MS/MS method for quantitative determination of Z-lumirubin

Popis výsledku anglicky

Although phototherapy (PT) is a standard treatment for neonatal jaundice, no validated clinical methods for determination of bilirubin phototherapy products are available. Thus, the aim of our study was to establish a such method for clinical use. To achieve this aim, a LC-MS/MS assay for simultaneous determination of Z-lumirubin (LR) and unconjugated bilirubin (UCB) was conducted. LR was purified after irradiation of UCB at 460 nm. The assay was tested on human sera from PT-treated neonates. Samples were separated on a HPLC system with a triple quadrupole mass spectrometer detector. The instrument response was linear up to 5.8 and 23.4 mg/dL for LR and UCB, respectively, with submicromolar limits of detection and validity parameters relevant for use in clinical medicine. Exposure of newborns to PT raised serum LR concentrations three-fold (p &lt; 0.01), but the absolute concentrations were low (0.37 +/- 0.16 mg/dL), despite a dramatic decrease of serum UCB concentrations (13.6 +/- 2.2 vs. 10.3 +/- 3.3 mg/dL, p &lt; 0.01). A LC-MS/MS method for the simultaneous determination of LR and UCB in human serum was established and validated for clinical use. This method should help to monitor neonates on PT, as well as to improve our understanding of both the kinetics and biology of bilirubin phototherapy products.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30209 - Paediatrics

Projekt

<a href="/cs/project/NV18-07-00342" target="_blank" >NV18-07-00342: Studium degradačních produktů bilirubinu vznikajících při fototerapii novorozenecké žloutenky</a><br>

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientific Reports

ISSN

2045-2322

e-ISSN

—

Svazek periodika

10

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

4411

Kód UT WoS článku

000563374600009

EID výsledku v databázi Scopus

2-s2.0-85081602730